During Muscle Atrophy, Thick, but not Thin, Filament Components are Degraded by MuRF1-Dependent Ubiquitylation

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During Muscle Atrophy, Thick, but not Thin, Filament Components are Degraded by MuRF1-Dependent Ubiquitylation

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Title: During Muscle Atrophy, Thick, but not Thin, Filament Components are Degraded by MuRF1-Dependent Ubiquitylation
Author: Brault, Jeffrey J.; Valenzuela, David M.; Gartner, Carlos; Latres, Esther; Cohen, Shenhav Orit; Gygi, Steven P.; Glass, David Jonathan; Goldberg, Alfred L.

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Citation: Cohen, Shenhav O., Jeffrey J. Brault, Steven P. Gygi, David J. Glass, David M. Valenzuela, Carlos Gartner, Esther Latres, and Alfred L. Goldberg. 2009. During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation. Journal of Cell Biology 185(6): 1083-1095.
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Abstract: Loss of myofibrillar proteins is a hallmark of atrophying muscle. Expression of muscle RING-finger 1 (MuRF1), a ubiquitin ligase, is markedly induced during atrophy, and MuRF1 deletion attenuates muscle wasting. We generated mice expressing a Ring-deletion mutant MuRF1, which binds but cannot ubiquitylate substrates. Mass spectrometry of the bound proteins in denervated muscle identified many myofibrillar components. Upon denervation or fasting, atrophying muscles show a loss of myosin-binding protein C (MyBP-C) and myosin light chains 1 and 2 (MyLC1 and MyLC2) from the myofibril, before any measurable decrease in myosin heavy chain (MyHC). Their selective loss requires MuRF1. MyHC is protected from ubiquitylation in myofibrils by associated proteins, but eventually undergoes MuRF1-dependent degradation. In contrast, MuRF1 ubiquitylates MyBP-C, MyLC1, and MyLC2, even in myofibrils. Because these proteins stabilize the thick filament, their selective ubiquitylation may facilitate thick filament disassembly. However, the thin filament components decreased by a mechanism not requiring MuRF1.
Published Version: doi:10.1083/jcb.200901052
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711608/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4706108

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