dc.contributor.author | Chen, Zhihong | |
dc.contributor.author | Ge, Yinlin | |
dc.contributor.author | Landman, Natalie | |
dc.contributor.author | Kang, Jing Xuan | |
dc.date.accessioned | 2011-02-21T16:41:27Z | |
dc.date.issued | 2002 | |
dc.identifier.citation | Chen, Zhihong, Yinlin Ge, Natalie Landman, and Jing X Kang. 2002. Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells. BMC Cancer 2: 18. | en_US |
dc.identifier.issn | 1471-2407 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728727 | |
dc.description.abstract | Background: Loss or mutation of the mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGF2R) has been found in breast cancer. However, whether or not decreased levels of functional M6P/IGF2R directly contribute to the process of carcinogenesis needs to be further verified by functional studies. Methods: In this study, using viral and ribozyme strategies we reduced the expression of M6P/IGF2R in human breast cancer cells and then examined the effect on growth and apoptosis of these cells. Results: Our results showed that infection of MCF-7 cells with the adenovirus carrying a ribozyme targeted against the M6P/IGF2R mRNA dramatically reduced the level of transcripts and the functional activity of M6P/IGF2R in these cells. Accordingly, cells treated with the ribozyme exhibited a higher growth rate and a lower apoptotic index than control cells (infected with a control vector). Furthermore, decreased expression of M6P/IGF2R enhanced IGF-II-induced proliferation and reduced cell susceptibility to TNF-induced apoptosis. Conclusions: These results suggest that M6P/IGF2R functions as a growth suppressor and its loss or mutation may contribute to development and progression of cancer. This study also demonstrates that adenoviral delivery of the ribozyme provides a useful tool for investigating the role of M6P/IGF2R in regulation of cell growth. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | doi://10.1186/1471-2407-2-18 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117795/pdf/ | en_US |
dash.license | LAA | |
dc.subject | tumor-necrosis-factor | en_US |
dc.subject | 6-Phosphate Receptor | en_US |
dc.subject | factor-alpha | en_US |
dc.subject | factor-beta | en_US |
dc.subject | suppressor gene | en_US |
dc.subject | growth-factor-2 receptor | en_US |
dc.subject | hepatocellular-carcinoma | en_US |
dc.subject | M6P/IGF2 receptor | en_US |
dc.subject | cathepsin-D | en_US |
dc.subject | carcinogenesis | en_US |
dc.title | Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | BMC Cancer | en_US |
dash.depositing.author | Kang, Jing Xuan | |
dc.date.available | 2011-02-21T16:41:27Z | |
dash.affiliation.other | HMS^Medicine-Massachusetts General Hospital | en_US |
dc.identifier.doi | 10.1186/1471-2407-2-18 | * |
dash.contributor.affiliated | Kang, Jing | |