Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells

Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells

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Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells

dc.contributor.author	Chen, Zhihong
dc.contributor.author	Ge, Yinlin
dc.contributor.author	Landman, Natalie
dc.contributor.author	Kang, Jing Xuan
dc.date.accessioned	2011-02-21T16:41:27Z
dc.date.issued	2002
dc.identifier.citation	Chen, Zhihong, Yinlin Ge, Natalie Landman, and Jing X Kang. 2002. Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells. BMC Cancer 2: 18.	en_US
dc.identifier.issn	1471-2407	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728727
dc.description.abstract	Background: Loss or mutation of the mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGF2R) has been found in breast cancer. However, whether or not decreased levels of functional M6P/IGF2R directly contribute to the process of carcinogenesis needs to be further verified by functional studies. Methods: In this study, using viral and ribozyme strategies we reduced the expression of M6P/IGF2R in human breast cancer cells and then examined the effect on growth and apoptosis of these cells. Results: Our results showed that infection of MCF-7 cells with the adenovirus carrying a ribozyme targeted against the M6P/IGF2R mRNA dramatically reduced the level of transcripts and the functional activity of M6P/IGF2R in these cells. Accordingly, cells treated with the ribozyme exhibited a higher growth rate and a lower apoptotic index than control cells (infected with a control vector). Furthermore, decreased expression of M6P/IGF2R enhanced IGF-II-induced proliferation and reduced cell susceptibility to TNF-induced apoptosis. Conclusions: These results suggest that M6P/IGF2R functions as a growth suppressor and its loss or mutation may contribute to development and progression of cancer. This study also demonstrates that adenoviral delivery of the ribozyme provides a useful tool for investigating the role of M6P/IGF2R in regulation of cell growth.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi://10.1186/1471-2407-2-18	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117795/pdf/	en_US
dash.license	LAA
dc.subject	tumor-necrosis-factor	en_US
dc.subject	6-Phosphate Receptor	en_US
dc.subject	factor-alpha	en_US
dc.subject	factor-beta	en_US
dc.subject	suppressor gene	en_US
dc.subject	growth-factor-2 receptor	en_US
dc.subject	hepatocellular-carcinoma	en_US
dc.subject	M6P/IGF2 receptor	en_US
dc.subject	cathepsin-D	en_US
dc.subject	carcinogenesis	en_US
dc.title	Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	BMC Cancer	en_US
dash.depositing.author	Kang, Jing Xuan
dc.date.available	2011-02-21T16:41:27Z
dash.affiliation.other	HMS^Medicine-Massachusetts General Hospital	en_US