Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells

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Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells

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dc.contributor.author Chen, Zhihong
dc.contributor.author Ge, Yinlin
dc.contributor.author Landman, Natalie
dc.contributor.author Kang, Jing Xuan
dc.date.accessioned 2011-02-21T16:41:27Z
dc.date.issued 2002
dc.identifier.citation Chen, Zhihong, Yinlin Ge, Natalie Landman, and Jing X Kang. 2002. Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells. BMC Cancer 2: 18. en_US
dc.identifier.issn 1471-2407 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728727
dc.description.abstract Background: Loss or mutation of the mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGF2R) has been found in breast cancer. However, whether or not decreased levels of functional M6P/IGF2R directly contribute to the process of carcinogenesis needs to be further verified by functional studies. Methods: In this study, using viral and ribozyme strategies we reduced the expression of M6P/IGF2R in human breast cancer cells and then examined the effect on growth and apoptosis of these cells. Results: Our results showed that infection of MCF-7 cells with the adenovirus carrying a ribozyme targeted against the M6P/IGF2R mRNA dramatically reduced the level of transcripts and the functional activity of M6P/IGF2R in these cells. Accordingly, cells treated with the ribozyme exhibited a higher growth rate and a lower apoptotic index than control cells (infected with a control vector). Furthermore, decreased expression of M6P/IGF2R enhanced IGF-II-induced proliferation and reduced cell susceptibility to TNF-induced apoptosis. Conclusions: These results suggest that M6P/IGF2R functions as a growth suppressor and its loss or mutation may contribute to development and progression of cancer. This study also demonstrates that adenoviral delivery of the ribozyme provides a useful tool for investigating the role of M6P/IGF2R in regulation of cell growth. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi://10.1186/1471-2407-2-18 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117795/pdf/ en_US
dash.license LAA
dc.subject tumor-necrosis-factor en_US
dc.subject 6-Phosphate Receptor en_US
dc.subject factor-alpha en_US
dc.subject factor-beta en_US
dc.subject suppressor gene en_US
dc.subject growth-factor-2 receptor en_US
dc.subject hepatocellular-carcinoma en_US
dc.subject M6P/IGF2 receptor en_US
dc.subject cathepsin-D en_US
dc.subject carcinogenesis en_US
dc.title Decreased expression of the mannose 6- phosphate/insulin-like growth factor-II receptor promotes growth of human breast cancer cells en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Cancer en_US
dash.depositing.author Kang, Jing Xuan
dc.date.available 2011-02-21T16:41:27Z
dash.affiliation.other HMS^Medicine-Massachusetts General Hospital en_US

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