Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno
| Title: | Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno |
| Author: |
Teerapittayanon, Surat; Vazquez, Alejandro; Douglas, Shawn Michael; Marblestone, Adam H.; Church, George McDonald; Shih, William
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Douglas, Shawn M., Adam H. Marblestone, Surat Teerapittayanon, Alejandro Vazquez, George M. Church, and William M. Shih. 2009. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Research 37(15): 5001-5006. |
| Full Text & Related Files: |
2731887.pdf (4.274Mb; PDF) 
|
| Abstract: | DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures, a long ‘scaffold’ strand can be employed to template the assembly of hundreds of oligonucleotide ‘staple’ strands into a planar antiparallel array of cross-linked helices. We recently adapted this ‘scaffolded DNA origami’ method to producing 3D shapes formed as pleated layers of double helices constrained to a honeycomb lattice. However, completing the required design steps can be cumbersome and time-consuming. Here we present caDNAno, an open-source software package with a graphical user interface that aids in the design of DNA sequences for folding 3D honeycomb-pleated shapes A series of rectangular-block motifs were designed, assembled, and analyzed to identify a well-behaved motif that could serve as a building block for future studies. The use of caDNAno significantly reduces the effort required to design 3D DNA-origami structures. The software is available at http://cadnano.org/, along with example designs and video tutorials demonstrating their construction. The source code is released under the MIT license. |
| Published Version: | doi:10.1093/nar/gkp436 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731887/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728739 |
This item appears in the following Collection(s)
-
HMS Scholarly Articles [4024]
Contact administrator regarding this item (to report mistakes or request changes)
