Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno

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Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno

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dc.contributor.author Teerapittayanon, Surat
dc.contributor.author Vazquez, Alejandro
dc.contributor.author Douglas, Shawn Michael
dc.contributor.author Marblestone, Adam H.
dc.contributor.author Church, George McDonald
dc.contributor.author Shih, William
dc.date.accessioned 2011-02-22T00:05:30Z
dc.date.issued 2009
dc.identifier.citation Douglas, Shawn M., Adam H. Marblestone, Surat Teerapittayanon, Alejandro Vazquez, George M. Church, and William M. Shih. 2009. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Research 37(15): 5001-5006. en_US
dc.identifier.issn 0305-1048 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728739
dc.description.abstract DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures, a long ‘scaffold’ strand can be employed to template the assembly of hundreds of oligonucleotide ‘staple’ strands into a planar antiparallel array of cross-linked helices. We recently adapted this ‘scaffolded DNA origami’ method to producing 3D shapes formed as pleated layers of double helices constrained to a honeycomb lattice. However, completing the required design steps can be cumbersome and time-consuming. Here we present caDNAno, an open-source software package with a graphical user interface that aids in the design of DNA sequences for folding 3D honeycomb-pleated shapes A series of rectangular-block motifs were designed, assembled, and analyzed to identify a well-behaved motif that could serve as a building block for future studies. The use of caDNAno significantly reduces the effort required to design 3D DNA-origami structures. The software is available at http://cadnano.org/, along with example designs and video tutorials demonstrating their construction. The source code is released under the MIT license. en_US
dc.language.iso en_US en_US
dc.publisher Oxford University Press en_US
dc.relation.isversionof doi:10.1093/nar/gkp436 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731887/pdf/ en_US
dash.license LAA
dc.title Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Nucleic Acids Research en_US
dash.depositing.author Douglas, Shawn Michael
dc.date.available 2011-02-22T00:05:30Z
dash.affiliation.other HMS^Genetics en_US
dash.affiliation.other HMS^Health Sciences and Technology en_US
dash.affiliation.other HMS^Genetics en_US
dash.affiliation.other HMS^Biological Chemistry and Molecular Pharmacology en_US

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