Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno

Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno

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Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno

dc.contributor.author	Teerapittayanon, Surat
dc.contributor.author	Vazquez, Alejandro
dc.contributor.author	Douglas, Shawn Michael
dc.contributor.author	Marblestone, Adam H.
dc.contributor.author	Church, George McDonald
dc.contributor.author	Shih, William
dc.date.accessioned	2011-02-22T00:05:30Z
dc.date.issued	2009
dc.identifier.citation	Douglas, Shawn M., Adam H. Marblestone, Surat Teerapittayanon, Alejandro Vazquez, George M. Church, and William M. Shih. 2009. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Research 37(15): 5001-5006.	en_US
dc.identifier.issn	0305-1048	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728739
dc.description.abstract	DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures, a long ‘scaffold’ strand can be employed to template the assembly of hundreds of oligonucleotide ‘staple’ strands into a planar antiparallel array of cross-linked helices. We recently adapted this ‘scaffolded DNA origami’ method to producing 3D shapes formed as pleated layers of double helices constrained to a honeycomb lattice. However, completing the required design steps can be cumbersome and time-consuming. Here we present caDNAno, an open-source software package with a graphical user interface that aids in the design of DNA sequences for folding 3D honeycomb-pleated shapes A series of rectangular-block motifs were designed, assembled, and analyzed to identify a well-behaved motif that could serve as a building block for future studies. The use of caDNAno significantly reduces the effort required to design 3D DNA-origami structures. The software is available at http://cadnano.org/, along with example designs and video tutorials demonstrating their construction. The source code is released under the MIT license.	en_US
dc.language.iso	en_US	en_US
dc.publisher	Oxford University Press	en_US
dc.relation.isversionof	doi:10.1093/nar/gkp436	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731887/pdf/	en_US
dash.license	LAA
dc.title	Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Nucleic Acids Research	en_US
dash.depositing.author	Douglas, Shawn Michael
dc.date.available	2011-02-22T00:05:30Z
dash.affiliation.other	HMS^Genetics	en_US
dash.affiliation.other	HMS^Health Sciences and Technology	en_US
dash.affiliation.other	HMS^Genetics	en_US
dash.affiliation.other	HMS^Biological Chemistry and Molecular Pharmacology	en_US