Beta-Synemin Expression in Cardiotoxin-Injected Rat Skeletal Muscle

Beta-Synemin Expression in Cardiotoxin-Injected Rat Skeletal Muscle

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Beta-Synemin Expression in Cardiotoxin-Injected Rat Skeletal Muscle

dc.contributor.author	Mizuno, Yuji
dc.contributor.author	Guyon, Jeffrey R
dc.contributor.author	Ishii, Akiko
dc.contributor.author	Hoshino, Sachiko
dc.contributor.author	Ohkoshi, Norio
dc.contributor.author	Tamaoka, Akira
dc.contributor.author	Okamoto, Koichi
dc.contributor.author	Kunkel, Louis Martens
dc.date.accessioned	2011-02-22T00:31:59Z
dc.date.issued	2007
dc.identifier.citation	Mizuno, Yuji, Jeffrey R. Guyon, Akiko Ishii, Sachiko Hoshino, Norio Ohkoshi, Akira Tamaoka, Koichi Okamoto, and Louis M. Kunkel. 2007. Beta-synemin expression in cardiotoxin-injected rat skeletal muscle. BMC Musculoskeletal Disorders 8: 40.	en_US
dc.identifier.issn	1471-2474	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728745
dc.description.abstract	Background: β-synemin was originally identified in humans as an α-dystrobrevin-binding protein through a yeast two-hybrid screen using an amino acid sequence derived from exons 1 through 16 of α-dystrobrevin, a region common to both α-dystrobrevin-1 and -2. α-Dystrobrevin-1 and -2 are both expressed in muscle and co-localization experiments have determined which isoform preferentially functions with β-synemin in vivo. The aim of our study is to show whether each α-dystrobrevin isoform has the same affinity for β-synemin or whether one of the isoforms preferentially functions with β-synemin in muscle. Methods: The two α-dystrobrevin isoforms (-1 and -2) and β-synemin were localized in regenerating rat tibialis anterior muscle using immunoprecipitation, immunohistochemical and immunoblot analyses. Immunoprecipitation and co-localization studies for α-dystrobrevin and β-synemin were performed in regenerating muscle following cardiotoxin injection. Protein expression was then compared to that of developing rat muscle using immunoblot analysis.Results: With an anti-α-dystrobrevin antibody, β-synemin co-immunoprecipitated with α-dystrobrevin whereas with an anti-β-synemin antibody, α-dystrobrevin-1 (rather than the -2 isoform) preferentially co-immunoprecipitated with β-synemin. Immunohistochemical experiments show that β-synemin and α-dystrobrevin co-localize in rat skeletal muscle. In regenerating muscle, β-synemin is first expressed at the sarcolemma and in the cytoplasm at day 5 following cardiotoxin injection. Similarly, β-synemin and α-dystrobrevin-1 are detected by immunoblot analysis as weak bands by day 7. In contrast, immunoblot analysis shows that α-dystrobrevin-2 is expressed as early as 1 day post-injection in regenerating muscle. These results are similar to that of developing muscle. For example, in embryonic rats, immunoblot analysis shows that β-synemin and α-dystrobevin-1 are weakly expressed in developing lower limb muscle at 5 days post-birth, while α-dystrobrevin-2 is detectable before birth in 20-day post-fertilization embryos. Conclusion: Our results clearly show that β-synemin expression correlates with that of α-dystrobrevin-1, suggesting that β-synemin preferentially functions with α-dystrobrevin-1 in vivo and that these proteins are likely to function coordinately to play a vital role in developing and regenerating muscle.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi:10.1186/1471-2474-8-40	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877804/pdf/	en_US
dash.license	LAA
dc.title	Beta-Synemin Expression in Cardiotoxin-Injected Rat Skeletal Muscle	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	BMC Musculoskeletal Disorders	en_US
dash.depositing.author	Kunkel, Louis Martens
dc.date.available	2011-02-22T00:31:59Z
dash.affiliation.other	HMS^Genetics	en_US
dash.affiliation.other	HMS^Pediatrics-Children's Hospital	en_US