Bioinformatic Identification and Characterization of Human Endothelial Cell-Restricted Genes

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Bioinformatic Identification and Characterization of Human Endothelial Cell-Restricted Genes

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dc.contributor.author Bhasin, Manoj
dc.contributor.author Oettgen, Peter
dc.contributor.author Yuan, Lei
dc.contributor.author Keskin, Derin Benerci
dc.contributor.author Otu, Hasan H
dc.contributor.author Libermann, Towia Aron
dc.date.accessioned 2011-02-22T17:36:15Z
dc.date.issued 2010
dc.identifier.citation Bhasin, Manoj, Lei Yuan, Derin B Keskin, Hasan H Otu, Towia A Libermann, and Peter Oettgen. 2010. Bioinformatic identification and characterization of human endothelial cell-restricted genes. BMC Genomics 11: 342. en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4729251
dc.description.abstract Background: In this study, we used a systematic bioinformatics analysis approach to elucidate genes that exhibit an endothelial cell (EC) restricted expression pattern, and began to define their regulation, tissue distribution, and potential biological role. Results: Using a high throughput microarray platform, a primary set of 1,191 transcripts that are enriched in different primary ECs compared to non-ECs was identified (LCB >3, FDR <2%). Further refinement of this initial subset of transcripts, using published data, yielded 152 transcripts (representing 109 genes) with different degrees of EC-specificity. Several interesting patterns emerged among these genes: some were expressed in all ECs and several were restricted to microvascular ECs. Pathway analysis and gene ontology demonstrated that several of the identified genes are known to be involved in vasculature development, angiogenesis, and endothelial function (P < 0.01). These genes are enriched in cardiovascular diseases, hemorrhage and ischemia gene sets (P < 0.001). Most of the identified genes are ubiquitously expressed in many different tissues. Analysis of the proximal promoter revealed the enrichment of conserved binding sites for 26 different transcription factors and analysis of the untranslated regions suggests that a subset of the EC-restricted genes are targets of 15 microRNAs. While many of the identified genes are known for their regulatory role in ECs, we have also identified several novel EC-restricted genes, the function of which have yet to be fully defined. Conclusion: The study provides an initial catalogue of EC-restricted genes most of which are ubiquitously expressed in different endothelial cells. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2164-11-342 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887814/pdf/ en_US
dash.license LAA
dc.title Bioinformatic Identification and Characterization of Human Endothelial Cell-Restricted Genes en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Genomics en_US
dash.depositing.author Otu, Hasan H
dc.date.available 2011-02-22T17:36:15Z
dash.affiliation.other HMS^Medicine- Beth Israel-Deaconess en_US
dash.affiliation.other HMS^Medicine- Beth Israel-Deaconess en_US

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