Comparative Transfection of DNA into Primary and Transformed Mammalian Cells From Different Lineages

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Comparative Transfection of DNA into Primary and Transformed Mammalian Cells From Different Lineages

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dc.contributor.author Maurisse, Rosalie
dc.contributor.author De Semir, David
dc.contributor.author Emamekhoo, Hamid
dc.contributor.author Abdolmohammadi, Alireza
dc.contributor.author Parsi, Hooman
dc.contributor.author Gruenert, Dieter C
dc.contributor.author Bedayat, Babak
dc.date.accessioned 2011-03-04T17:53:05Z
dc.date.issued 2010
dc.identifier.citation Maurisse, Rosalie, David De Semir, Hamid Emamekhoo, Babak Bedayat, Alireza Abdolmohammadi, Hooman Parsi, and Dieter C Gruenert. 2010. Comparative transfection of DNA into primary and transformed mammalian cells from different lineages. BMC Biotechnology 10: 9. en_US
dc.identifier.issn 1472-6750 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4737684
dc.description.abstract Background: The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their efficacy has been variable and is highly dependent on the cell type to be transfected. Results: Studies were undertaken to evaluate and compare the transfection efficacy of several chemical reagents to that of the electroporation/nucleofection system using both adherent cells (primary and transformed airway epithelial cells and primary fibroblasts as well as embryonic stem cells) and cells in suspension (primary hematopoietic stem/progenitor cells and lymphoblasts). With the exception of HEK 293 cell transfection, nucleofection proved to be less toxic and more efficient at effectively delivering DNA into the cells as determined by cell proliferation and GFP expression, respectively. Lipofectamine and nucleofection of HEK 293 were essentially equivalent in terms of toxicity and efficiency. Transient transfection efficiency in all the cell systems ranged from 40%-90%, with minimal toxicity and no apparent species specificity. Differences in efficiency and toxicity were cell type/system specific. Conclusions: In general, the Amaxa electroporation/nucleofection system appears superior to other chemical systems. However, there are cell-type and species specific differences that need to be evaluated empirically to optimize the conditions for transfection efficiency and cell survival. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi://10.1186/1472-6750-10-9 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830169/pdf/ en_US
dash.license LAA
dc.title Comparative Transfection of DNA into Primary and Transformed Mammalian Cells From Different Lineages en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Biotechnology en_US
dash.depositing.author Bedayat, Babak
dc.date.available 2011-03-04T17:53:05Z
dash.affiliation.other HMS^Anaesthesia-Massachusetts General Hospital en_US

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