Identifying Genes Preferentially Expressed in Undifferentiated Embryonic Stem Cells

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Identifying Genes Preferentially Expressed in Undifferentiated Embryonic Stem Cells

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dc.contributor.author Leder, Philip
dc.contributor.author Li, Xiajun
dc.date.accessioned 2011-03-06T02:16:22Z
dc.date.issued 2007
dc.identifier.citation Li, Xiajun, and Philip Leder. 2007. Identifying genes preferentially expressed in undifferentiated embryonic stem cells. BMC Cell Biology 8: 37. en_US
dc.identifier.issn 1471-2121 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4738025
dc.description.abstract Background: The mechanism involved in the maintenance and differentiation of embryonic stem (ES) cells is incompletely understood. Results: To address this issue, we have developed a retroviral gene trap vector that can target genes expressed in undifferentiated ES cells. This gene trap vector harbors both GFP and Neo reporter genes. G-418 drug resistance was used to select ES clones in which the vector was integrated into transcriptionally active loci. This was then followed by GFP FACS profiling to identify ES clones with reduced GFP fluorescence and, hence, reduced transcriptional activity when ES cells differentiate. Reduced expression of the GFP reporter in six of three hundred ES clones in our pilot screening was confirmed to be down-regulated by Northern blot analysis during ES cell differentiation. These six ES clones represent four different genes. Among the six integration sites, one was at Zfp-57 whose gene product is known to be enriched in undifferentiated ES cells. Three were located in an intron of a novel isoform of CSL/RBP-Jkappa which encodes the key transcription factor of the LIN-12/Notch pathway. Another was inside a gene that may encode noncoding RNA transcripts. The last integration event occurred at a locus that may harbor a novel gene. Conclusion: Taken together, we demonstrate the use of a novel retroviral gene trap vector in identifying genes preferentially expressed in undifferentiated ES cells. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2121-8-37 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1995199/pdf/ en_US
dash.license LAA
dc.title Identifying Genes Preferentially Expressed in Undifferentiated Embryonic Stem Cells en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Cell Biology en_US
dash.depositing.author Leder, Philip
dc.date.available 2011-03-06T02:16:22Z
dash.affiliation.other HMS^Medicine- Beth Israel-Deaconess en_US
dash.affiliation.other HMS^Emeritus en_US

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