Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution

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Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution

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dc.contributor.author Long, Tao
dc.contributor.author Tu, Kimberly C
dc.contributor.author Ong, N. P
dc.contributor.author Bassler, Bonnie L
dc.contributor.author Wingreen, Ned S
dc.contributor.author Wang, Yufang
dc.contributor.author Mehta, Pankaj
dc.contributor.author Kishony, Roy
dc.date.accessioned 2011-03-08T18:29:07Z
dc.date.issued 2009
dc.identifier.citation Long, Tao, Kimberly C Tu, Yufang Wang, Pankaj Mehta, N. P Ong, Bonnie L Bassler, and Ned S Wingreen. 2009. Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution. PLoS Biology 7(3). en_US
dc.identifier.issn 1544-9173 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4739285
dc.description.abstract Cell-to-cell communication in bacteria is a process known as quorum sensing that relies on the production, detection, and response to the extracellular accumulation of signaling molecules called autoinducers. Often, bacteria use multiple autoinducers to obtain information about the vicinal cell density. However, how cells integrate and interpret the information contained within multiple autoinducers remains a mystery. Using single-cell fluorescence microscopy, we quantified the signaling responses to and analyzed the integration of multiple autoinducers by the model quorum-sensing bacterium Vibrio harveyi. Our results revealed that signals from two distinct autoinducers, AI-1 and AI-2, are combined strictly additively in a shared phosphorelay pathway, with each autoinducer contributing nearly equally to the total response. We found a coherent response across the population with little cell-to-cell variation, indicating that the entire population of cells can reliably distinguish several distinct conditions of external autoinducer concentration. We speculate that the use of multiple autoinducers allows a growing population of cells to synchronize gene expression during a series of distinct developmental stages. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi://10.1371/journal.pbio.1000068 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2661960/pdf/ en_US
dash.license LAA
dc.subject microbiology en_US
dc.subject biophysics en_US
dc.title Quantifying the Integration of Quorum-Sensing Signals with Single-Cell Resolution en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Biology en_US
dash.depositing.author Wang, Yufang
dc.date.available 2011-03-08T18:29:07Z
dash.affiliation.other HMS^Pathology en_US

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