Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy

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Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy

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dc.contributor.author Ohi, Melanie
dc.contributor.author Cheng, Yifan
dc.contributor.author Li, Ying
dc.contributor.author Walz, Thomas
dc.date.accessioned 2011-03-15T20:33:51Z
dc.date.issued 2004
dc.identifier.citation Ohi, Melanie, Ying Li, Yifan Cheng, and Thomas Walz. 2004. Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy. Biological Procedures Online 6(1): 23-34. en_US
dc.identifier.issn 1480-9222 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4742426
dc.description.abstract Vitrification is the state-of-the-art specimen preparation technique for molecular electron microscopy (EM) and therefore negative staining may appear to be an outdated approach. In this paper we illustrate the specific advantages of negative staining, ensuring that this technique will remain an important tool for the study of biological macromolecules. Due to the higher image contrast, much smaller molecules can be visualized by negative staining. Also, while molecules prepared by vitrification usually adopt random orientations in the amorphous ice layer, negative staining tends to induce preferred orientations of the molecules on the carbon support film. Combining negative staining with image classification techniques makes it possible to work with very heterogeneous molecule populations, which are difficult or even impossible to analyze using vitrified specimens. en_US
dc.language.iso en_US en_US
dc.publisher Biological Procedures Online en_US
dc.relation.isversionof doi:10.1251/bpo70 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC389902/pdf/ en_US
dash.license LAA
dc.title Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Biological Procedures Online en_US
dash.depositing.author Walz, Thomas
dc.date.available 2011-03-15T20:33:51Z
dash.affiliation.other HMS^Cell Biology en_US
dash.affiliation.other HMS^Cell Biology en_US

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