A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses

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A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses

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dc.contributor.author Berger, Cedric R.
dc.contributor.author Figueiredo, Jose-Luiz
dc.contributor.author Swirski, Filip K.
dc.contributor.author Mempel, Thorsten Roman
dc.contributor.author Von Andrian, Ulrich H.
dc.contributor.author Pittet, Mikael J.
dc.contributor.author Weissleder, Ralph
dc.date.accessioned 2011-03-19T01:08:22Z
dc.date.issued 2007
dc.identifier.citation Swirski, Filip K., Cedric R. Berger, Jose-Luiz Figueiredo, Thorsten R. Mempel, Ulrich H. von Andrian, Mikael J. Pittet, and Ralph Weissleder. 2007. A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses. PLoS ONE 2(10): e1075. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4745724
dc.description.abstract The complexity of the tumor microenvironment necessitates that cell behavior is studied in a broad, multi-scale context. Although tomographic and microscopy-based far and near infrared fluorescence (NIRF, >650 nm) imaging methods offer high resolution, sensitivity, and depth penetration, there has been a lack of optimized NIRF agents to label and track cells in their native environments at different scales. In this study we labeled mammalian leukocytes with VivoTag 680 (VT680), an amine reactive N-hydroxysuccinimide (NHS) ester of a (benz) indolium-derived far red fluorescent probe. We show that VT680 diffuses into leukocytes within minutes, covalently binds to cellular components, remains internalized for days in vitro and in vivo, and does not transfer fluorescence to adjacent cells. It is biocompatible, keeps cells fully functional, and fluoresces at high intensities. In a tumor model of cytotoxic T lymphocyte (CTL) immunotherapy, we track and quantify VT680-labeled cells longitudinally at the whole-body level with fluorescence-mediated molecular tomography (FMT), within tissues at single cell resolutions by multiphoton and confocal intravital microscopy, and ex vivo by flow cytometry. Thus, this approach is suitable to monitor cells at multiple resolutions in real time in their native environments by NIR-based fluorescence imaging. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0001075 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034600/pdf/ en_US
dash.license LAA
dc.subject radiology and medical imaging en_US
dc.subject immunology en_US
dc.subject immune response en_US
dc.subject immunomodulation en_US
dc.subject leukocyte activation en_US
dc.title A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Swirski, Filip K.
dc.date.available 2011-03-19T01:08:22Z
dash.affiliation.other HMS^Radiology-Massachusetts General Hospital en_US
dash.affiliation.other HMS^Radiology-Massachusetts General Hospital en_US
dash.affiliation.other HMS^Radiology-Massachusetts General Hospital en_US
dash.affiliation.other HMS^Systems Biology en_US

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