The Vibrio harveyi Master Quorum-sensing Regulator, LuxR, a TetR-type Protein is Both an Activator and a Repressor: DNA Recognition and Binding Specificity at Target Promoters

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The Vibrio harveyi Master Quorum-sensing Regulator, LuxR, a TetR-type Protein is Both an Activator and a Repressor: DNA Recognition and Binding Specificity at Target Promoters

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dc.contributor.author Pompeani, Audra J
dc.contributor.author Irgon, Joseph J
dc.contributor.author Berger, Michael F
dc.contributor.author Wingreen, Ned S
dc.contributor.author Bassler, Bonnie L
dc.contributor.author Bulyk, Martha Leonia
dc.date.accessioned 2011-03-19T06:30:14Z
dc.date.issued 2008
dc.identifier.citation Pompeani, Audra J., Joseph J. Irgon, Michael F. Berger, Martha L. Bulyk, Ned S. Wingreen, and Bonnie L. Bassler. 2008. The Vibrio harveyi master quorum-sensing regulator, LuxR, a TetR-type protein is both an activator and a repressor: DNA recognition and binding specificity at target promoters. Molecular Microbiology 70(1): 76-88. en_US
dc.identifier.issn 0950-382X en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4745731
dc.description.abstract Quorum sensing is the process of cell-to-cell communication by which bacteria communicate via secreted signal molecules called autoinducers. As cell population density increases, the accumulation of autoinducers leads to co-ordinated changes in gene expression across the bacterial community. The marine bacterium, Vibrio harveyi, uses three autoinducers to achieve intra-species, intra-genera and inter-species cell–cell communication. The detection of these autoinducers ultimately leads to the production of LuxR, the quorum-sensing master regulator that controls expression of the genes in the quorum-sensing regulon. LuxR is a member of the TetR protein superfamily; however, unlike other TetR repressors that typically repress their own gene expression and that of an adjacent operon, LuxR is capable of activating and repressing a large number of genes. Here, we used protein binding microarrays and a two-layered bioinformatics approach to show that LuxR binds a 21 bp consensus operator with dyad symmetry. In vitro and in vivo analyses of two promoters directly regulated by LuxR allowed us to identify those bases that are critical for LuxR binding. Together, the in silico and biochemical results enabled us to scan the genome and identify novel targets of LuxR in V. harveyi and thus expand the understanding of the quorum-sensing regulon. en_US
dc.language.iso en_US en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isversionof doi:10.1111/j.1365-2958.2008.06389.x en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628434/pdf/ en_US
dash.license LAA
dc.title The Vibrio harveyi Master Quorum-sensing Regulator, LuxR, a TetR-type Protein is Both an Activator and a Repressor: DNA Recognition and Binding Specificity at Target Promoters en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Molecular Microbiology en_US
dash.depositing.author Bulyk, Martha Leonia
dc.date.available 2011-03-19T06:30:14Z
dash.affiliation.other HMS^Pathology en_US
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US

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