Dendritic Cells are Preferentially Targeted among Hematolymphocytes by Modified Vaccinia Virus Ankara and Play a Key Role in the Induction of Virus-Specific T Cell Responses In Vivo

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Dendritic Cells are Preferentially Targeted among Hematolymphocytes by Modified Vaccinia Virus Ankara and Play a Key Role in the Induction of Virus-Specific T Cell Responses In Vivo

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dc.contributor.author Chavan, Rahul
dc.contributor.author Liu, Luzheng
dc.contributor.author Feinberg, Mark B.
dc.date.accessioned 2011-03-22T16:38:31Z
dc.date.issued 2008
dc.identifier.citation Liu, Luzheng, Rahul Chavan, and Mark B, Feinberg. 2008. Dendritic Cells are preferentially targeted among hematolymphocytes by Modified Vaccinia Virus Ankara and play a key role in the induction of virus-specific T cell responses in vivo. BMC Immunology 9: 15. en_US
dc.identifier.issn 1471-2172 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4750996
dc.description.abstract Background: Modified Vaccinia Ankara (MVA) is a highly attenuated strain of vaccinia virus (VV) that has lost approximately 15% of the VV genome, along with the ability to replicate in most mammalian cells. It has demonstrated impressive safety and immunogenicity profile in both preclinical and clinical studies, and is being actively explored as a promising vaccine vector for a number of infectious diseases and malignancies. However, little is known about how MVA interacts with the host immune system constituents, especially dendritic cells (DCs), to induce strong immune responses despite its inability to replicate in vivo. Using in vitro and in vivo murine models, we systematically investigated the susceptibility of murine DCs to MVA infection, and the immunological consequences of the infection. Results: Our data demonstrate that MVA preferentially infects professional antigen presenting cells, especially DCs, among all the subsets of hematolymphoid cells. In contrast to the reported blockage of DC maturation and function upon VV infection, DCs infected by MVA undergo phenotypic maturation and produce innate cytokine IFN-α within 18 h of infection. Substantial apoptosis of MVA-infected DCs occurs after 12 h following infection and the apoptotic DCs are readily phagocytosed by uninfected DCs. Using MHC class I – deficient mice, we showed that both direct and cross-presentation of viral Ags are likely to be involved in generating viral-specific CD8+ T cell responses. Finally, DC depletion abrogated the T cell activation in vivo. Conclusion: We present the first in vivo evidence that among hematolymphoid cells, DCs are the most susceptible targets for MVA infection, and DC-mediated Ag presentation is required for the induction of MVA-specific immune responses. These results provide important information concerning the mechanisms by which strong immune responses are elicited to MVA-encoded antigens and may inform efforts to further improve the immunogenicity of this already promising vaccine vector. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2172-9-15 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2359732/pdf/ en_US
dash.license LAA
dc.title Dendritic Cells are Preferentially Targeted among Hematolymphocytes by Modified Vaccinia Virus Ankara and Play a Key Role in the Induction of Virus-Specific T Cell Responses In Vivo en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Immunology en_US
dash.depositing.author Liu, Luzheng
dc.date.available 2011-03-22T16:38:31Z
dash.affiliation.other HMS^Dermatology-Brigham and Women's Hospital en_US

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