Heritable Stochastic Switching Revealed by Single-Cell Genealogy

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Heritable Stochastic Switching Revealed by Single-Cell Genealogy

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dc.contributor.author Yang, Qiong
dc.contributor.author Mettetal, Jerome T
dc.contributor.author van Oudenaarden, Alexander
dc.contributor.author Kaufmann, Benjamin Bailey
dc.date.accessioned 2011-03-30T16:02:13Z
dc.date.issued 2007
dc.identifier.citation Kaufmann, Benjamin B., Qiong Yang, Jerome T. Mettetal, and Alexander van Oudenaarden. 2007. Heritable stochastic switching revealed by single-cell genealogy. PLoS Biology 5(9): e239. en_US
dc.identifier.issn 1544-9173 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4782109
dc.description.abstract The partitioning and subsequent inheritance of cellular factors like proteins and RNAs is a ubiquitous feature of cell division. However, direct quantitative measures of how such nongenetic inheritance affects subsequent changes in gene expression have been lacking. We tracked families of the yeast Saccharomyces cerevisiae as they switch between two semi-stable epigenetic states. We found that long after two cells have divided, they continued to switch in a synchronized manner, whereas individual cells have exponentially distributed switching times. By comparing these results to a Poisson process, we show that the time evolution of an epigenetic state depends initially on inherited factors, with stochastic processes requiring several generations to decorrelate closely related cells. Finally, a simple stochastic model demonstrates that a single fluctuating regulatory protein that is synthesized in large bursts can explain the bulk of our results. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pbio.0050239 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964776/pdf/ en_US
dash.license LAA
dc.subject biophysics en_US
dc.subject genetics and genomics en_US
dc.subject mathematics en_US
dc.subject yeast and fungi en_US
dc.title Heritable Stochastic Switching Revealed by Single-Cell Genealogy en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Biology en_US
dash.depositing.author Kaufmann, Benjamin Bailey
dc.date.available 2011-03-30T16:02:13Z
dash.affiliation.other HMS^Microbiology and Molecular Genetics en_US

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