c-Fms-Mediated Differentiation and Priming of Monocyte Lineage Cells Play a Central Role in Autoimmune Arthritis

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c-Fms-Mediated Differentiation and Priming of Monocyte Lineage Cells Play a Central Role in Autoimmune Arthritis

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dc.contributor.author Paniagua, Ricardo T
dc.contributor.author Chang, Anna
dc.contributor.author Mariano, Melissa M
dc.contributor.author Stein, Emily A
dc.contributor.author Lindstrom, Tamsin M
dc.contributor.author Sharpe, Orr
dc.contributor.author Roscow, Claire
dc.contributor.author Ho, Peggy P
dc.contributor.author Wang, Qian
dc.contributor.author Lee, David Marvin
dc.contributor.author Robinson, William H.
dc.date.accessioned 2011-03-30T16:30:24Z
dc.date.issued 2010
dc.identifier.citation Paniagua, Ricardo T., Anna Chang, Melissa M. Mariano, Emily A. Stein, Qian Wang, Tamsin M. Lindstrom, Orr Sharpe, et al. 2010. c-Fms-mediated differentiation and priming of monocyte lineage cells play a central role in autoimmune arthritis. Arthritis Research & Therapy 12(1): R32. en_US
dc.identifier.issn 1478-6354 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4782727
dc.description.abstract Introduction: Tyrosine kinases are key mediators of multiple signaling pathways implicated in rheumatoid arthritis (RA). We previously demonstrated that imatinib mesylate--a Food and Drug Administration (FDA)-approved, antineoplastic drug that potently inhibits the tyrosine kinases Abl, c-Kit, platelet-derived growth factor receptor (PDGFR), and c-Fms--ameliorates murine autoimmune arthritis. However, which of the imatinib-targeted kinases is the principal culprit in disease pathogenesis remains unknown. Here we examine the role of c-Fms in autoimmune arthritis. Methods: We tested the therapeutic efficacy of orally administered imatinib or GW2580, a small molecule that specifically inhibits c-Fms, in three mouse models of RA: collagen-induced arthritis (CIA), anti-collagen antibody-induced arthritis (CAIA), and K/BxN serum transfer-induced arthritis (K/BxN). Efficacy was evaluated by visual scoring of arthritis severity, paw thickness measurements, and histological analysis. We assessed the in vivo effects of imatinib and GW2580 on macrophage infiltration of synovial joints in CIA, and their in vitro effects on macrophage and osteoclast differentiation, and on osteoclast-mediated bone resorption. Further, we determined the effects of imatinib and GW2580 on the ability of macrophage colony-stimulating factor (M-CSF; the ligand for c-Fms) to prime bone marrow-derived macrophages to produce tumor necrosis factor (TNF) upon subsequent Fc receptor ligation. Finally, we measured M-CSF levels in synovial fluid from patients with RA, osteoarthritis (OA), or psoriatic arthritis (PsA), and levels of total and phosphorylated c-Fms in synovial tissue from patients with RA. Results: GW2580 was as efficacious as imatinib in reducing arthritis severity in CIA, CAIA, and K/BxN models of RA. Specific inhibition of c-Fms abrogated (i) infiltration of macrophages into synovial joints of arthritic mice; (ii) differentiation of monocytes into macrophages and osteoclasts; (iii) osteoclast-mediated bone resorption; and (iv) priming of macrophages to produce TNF upon Fc receptor stimulation, an important trigger of synovitis in RA. Expression and activation of c-Fms in RA synovium were high, and levels of M-CSF were higher in RA synovial fluid than in OA or PsA synovial fluid. Conclusions: These results suggest that c-Fms plays a central role in the pathogenesis of RA by mediating the differentiation and priming of monocyte lineage cells. Therapeutic targeting of c-Fms could provide benefit in RA. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/ar2940 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875666/pdf/ en_US
dash.license LAA
dc.title c-Fms-Mediated Differentiation and Priming of Monocyte Lineage Cells Play a Central Role in Autoimmune Arthritis en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Arthritis Research & Therapy en_US
dash.depositing.author Lee, David Marvin
dc.date.available 2011-03-30T16:30:24Z
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US

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