Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low–Birth Weight Mice

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Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low–Birth Weight Mice

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dc.contributor.author Jimenez-Chillaron, Jose
dc.contributor.author Chow, Alice
dc.contributor.author DeCoste, Jennifer
dc.contributor.author Vokes, Martha
dc.contributor.author Liu, Manway
dc.contributor.author Kasif, Simon
dc.contributor.author Zavacki, Ann-Marie
dc.contributor.author Leshan, Rebecca L.
dc.contributor.author Myers, Martin G.
dc.contributor.author Isganaitis, Elvira
dc.contributor.author Woo, Melissa
dc.contributor.author Patti, Mary-Elizabeth
dc.date.accessioned 2011-04-08T16:10:42Z
dc.date.issued 2009
dc.identifier.citation Isganaitis, Elvira, Jose Jimenez-Chillaron, Melissa Woo, Alice Chow, Jennifer DeCoste, Martha Vokes, Manway Liu, et al. 2009. Accelerated postnatal growth increases lipogenic gene expression and adipocyte size in low-birth weight mice. Diabetes 58(5): 1192-1200. en_US
dc.identifier.issn 0012-1797 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4817606
dc.description.abstract OBJECTIVE: To characterize the hormonal milieu and adipose gene expression in response to catch-up growth (CUG), a growth pattern associated with obesity and diabetes risk, in a mouse model of low birth weight (LBW). RESEARCH DESIGN AND METHODS: ICR mice were food restricted by 50% from gestational days 12.5–18.5, reducing offspring birth weight by 25%. During the suckling period, dams were either fed ad libitum, permitting CUG in offspring, or food restricted, preventing CUG. Offspring were killed at age 3 weeks, and gonadal fat was removed for RNA extraction, array analysis, RT-PCR, and evaluation of cell size and number. Serum insulin, thyroxine (T4), corticosterone, and adipokines were measured. RESULTS: At age 3 weeks, LBW mice with CUG (designated U-C) had body weight comparable with controls (designated C-C); weight was reduced by 49% in LBW mice without CUG (designated U-U). Adiposity was altered by postnatal nutrition, with gonadal fat increased by 50% in U-C and decreased by 58% in U-U mice (P less than 0.05 vs. C-C mice). Adipose expression of the lipogenic genes Fasn, AccI, Lpin1, and Srebf1 was significantly increased in U-C compared with both C-C and U-U mice (P less than 0.05). Mitochondrial DNA copy number was reduced by greater than 50% in U-C versus U-U mice (P = 0.014). Although cell numbers did not differ, mean adipocyte diameter was increased in U-C and reduced in U-U mice (P less than 0.01). CONCLUSIONS: CUG results in increased adipose tissue lipogenic gene expression and adipocyte diameter but not increased cellularity, suggesting that catch-up fat is primarily associated with lipogenesis rather than adipogenesis in this murine model. en_US
dc.language.iso en_US en_US
dc.publisher American Diabetes Association en_US
dc.relation.isversionof doi:10.2337/db08-1266 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671035/pdf/ en_US
dash.license META_ONLY
dc.subject Pathophysiology en_US
dc.title Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low–Birth Weight Mice en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Diabetes en_US
dash.depositing.author Isganaitis, Elvira
dash.embargo.until 10000-01-01
dash.affiliation.other HMS^Pediatrics-Massachusetts General Hospital en_US

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