dc.contributor.author | Vermeersch, Pieter | |
dc.contributor.author | Pokreisz, Peter | |
dc.contributor.author | Marsboom, Glenn | |
dc.contributor.author | Gillijns, Hilde | |
dc.contributor.author | Pellens, Marijke | |
dc.contributor.author | Dewerchin, Mieke | |
dc.contributor.author | Brouckaert, Peter | |
dc.contributor.author | Janssens, Stefan | |
dc.contributor.author | Buys, Emmanuel | |
dc.contributor.author | Sips, Patrick | |
dc.contributor.author | Bloch, Kenneth Daniel | |
dc.date.accessioned | 2011-04-08T16:22:18Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Vermeersch, Pieter, Emmanuel Buys, Patrick Sips, Peter Pokreisz, Glenn Marsboom, Hilde Gillijns, Marijke Pellens, and et al. 2009. Gender-specific modulation of the response to arterial injury by soluble guanylate cyclase α1. The Open Cardiovascular Medicine Journal 3: 98-104. | en_US |
dc.identifier.issn | 1874-1924 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4817626 | |
dc.description.abstract | Objective: Soluble guanylate cyclase (sGC), a heterodimer composed of α and β subunits, synthesizes cGMP in response to nitric oxide (NO). NO modulates vascular tone and structure but the relative contributions of cGMP-dependent versus cGMP-independent mechanisms remain uncertain. We studied the response to vascular injury in male (M) and female (F) mice with targeted deletion of exon 6 of the sGCα1 subunit (sGCα1-/-), resulting in a non-functional heterodimer. Methods: We measured aortic cGMP levels and mRNA transcripts encoding sGC α1, α2, and β1 subunits in wild type (WT) and sGCa1-/- mice. To study the response to vascular injury, BrdU-incorporation and neointima formation (maximum intima to media (I/M) ratio) were determined 5 and 28 days after carotid artery ligation, respectively. Results: Aortic cGMP levels were 4-fold higher in F than in M mice in both genotypes, and, within each gender, 4-fold higher in WT than in sGCa1-/-. In contrast, sGCα1, sGCα2, and sGCβ1 mRNA expression did not differ between groups. 3H-thymidine incorporation in cultured sGCa1-/- smooth muscle cells (SMC) was 27%±12% lower than in WT SMC and BrdU-incorporation in carotid arteries 5 days after ligation was significantly less in sGCa1-/- M than in WT M. Neointima area and I/M 28 days after ligation were 65% and 62% lower in sGCa1-/- M than in WT M mice (p<0,05 for both) but were not different in F mice. Conclusion: Functional deletion of sGCa1 resulted in reduced cGMP levels in male sGCa1-/- mice and a gender-specific effect on the adaptive response to vascular injury. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Bentham Open | en_US |
dc.relation.isversionof | doi://10.2174/1874192400903010098 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743853/pdf/ | en_US |
dash.license | LAA | |
dc.subject | guanylate cyclase | en_US |
dc.subject | gender | en_US |
dc.subject | vascular remodelling | en_US |
dc.subject | citric oxide | en_US |
dc.title | Gender-Specific Modulation of the Response to Arterial Injury by Soluble Guanylate Cyclase α1 | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | The Open Cardiovascular Medicine Journal | en_US |
dash.depositing.author | Buys, Emmanuel | |
dc.date.available | 2011-04-08T16:22:18Z | |
dash.affiliation.other | HMS^Anaesthesia-Massachusetts General Hospital | en_US |
dc.identifier.doi | 10.2174/1874192400903010098 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Sips, Patrick | |
dash.contributor.affiliated | Bloch, Kenneth | |
dash.contributor.affiliated | Buys, Emmanuel | |