Identification of Molecular Markers of Bipolar Cells in the Murine Retina

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Identification of Molecular Markers of Bipolar Cells in the Murine Retina

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Title: Identification of Molecular Markers of Bipolar Cells in the Murine Retina
Author: Kim, Douglas S; Trimarchi, Jeffrey M; Ross, Sarah Elizabeth; Aach, John Dennis; Greenberg, Michael Eldon; Cepko, Connie

Note: Order does not necessarily reflect citation order of authors.

Citation: Kim, Douglas S., Sarah E. Ross, Jeffrey M. Trimarchi, John Aach, Michael E. Greenberg, and Constance L. Cepko. 2008. Identification of molecular markers of bipolar cells in the murine retina. The Journal of Comparative Neurology 507(5): 1795-1810.
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Abstract: Retinal bipolar neurons serve as relay interneurons that connect rod and cone photoreceptor cells to amacrine and ganglion cells. They exhibit diverse morphologies essential for correct routing of photoreceptor cell signals to specific postsynaptic amacrine and ganglion cells. The development and physiology of these interneurons have not been completely defined molecularly. Despite previous identification of genes expressed in several bipolar cell subtypes, molecules that mark each bipolar cell type still await discovery. In this report, novel genetic markers of murine bipolar cells were found. Candidates were initially generated by using microarray analysis of single bipolar cells and mining of retinal serial analysis of gene expression (SAGE) data. These candidates were subsequently tested for expression in bipolar cells by RNA in situ hybridization. Ten new molecular markers were identified, five of which are highly enriched in their expression in bipolar cells within the adult retina. Double-labeling experiments using probes for previously characterized subsets of bipolar cells were performed to identify the subtypes of bipolar cells that express the novel markers. Additionally, the expression of bipolar cell genes was analyzed in Bhlhb4 knockout retinas, in which rod bipolar cells degenerate postnatally, to delineate further the identity of bipolar cells in which novel markers are found. From the analysis of Bhlhb4 mutant retinas, cone bipolar cell gene expression appears to be relatively unaffected by the degeneration of rod bipolar cells. Identification of molecular markers for the various subtypes of bipolar cells will lead to greater insights into the development and function of these diverse interneurons.
Published Version: doi:10.1002/cne.21639
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665264/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4851330

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