The Uncoupling Protein 1 Gene, UCP1, is Expressed in Mammalian Islet Cells and Associated with Acute Insulin Response to Glucose in African American Families from the IRAS Family Study

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The Uncoupling Protein 1 Gene, UCP1, is Expressed in Mammalian Islet Cells and Associated with Acute Insulin Response to Glucose in African American Families from the IRAS Family Study

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dc.contributor.author Sale, Michèle M
dc.contributor.author Hsu, Fang-Chi
dc.contributor.author Palmer, Nicholette D
dc.contributor.author Gordon, Candace J
dc.contributor.author Keene, Keith L
dc.contributor.author Borgerink, Hermina M
dc.contributor.author Bergman, Richard N
dc.contributor.author Taylor, Kent D
dc.contributor.author Saad, Mohammed F
dc.contributor.author Norris, Jill M
dc.contributor.author Sharma, Arun J.
dc.date.accessioned 2011-04-16T01:58:07Z
dc.date.issued 2007
dc.identifier.citation Sale, Michéle M, Fang-Chi Hsu, Nicholette D Palmer, Candace J Gordon, Keith L Keene, Hermina M Borgerink, Arun J Sharma, and et al. 2007. The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study. BMC Endocrine Disorders 7: 1. en_US
dc.identifier.issn 1472-6823 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4851339
dc.description.abstract Background: Variants of uncoupling protein genes UCP1 and UCP2 have been associated with a range of traits. We wished to evaluate contributions of known UCP1 and UCP2 variants to metabolic traits in the Insulin Resistance and Atherosclerosis (IRAS) Family Study. Methods: We genotyped five promoter or coding single nucleotide polymorphisms (SNPs) in 239 African American (AA) participants and 583 Hispanic participants from San Antonio (SA) and San Luis Valley. Generalized estimating equations using a sandwich estimator of the variance and exchangeable correlation to account for familial correlation were computed for the test of genotypic association, and dominant, additive and recessive models. Tests were adjusted for age, gender and BMI (glucose homeostasis and lipid traits), or age and gender (obesity traits), and empirical P-values estimated using a gene dropping approach. Results: UCP1 A-3826G was associated with AIRg in AA (P = 0.006) and approached significance in Hispanic families (P = 0.054); and with HDL-C levels in SA families (P = 0.0004). Although UCP1 expression is reported to be restricted to adipose tissue, RT-PCR indicated that UCP1 is expressed in human pancreas and MIN-6 cells, and immunohistochemistry demonstrated co-localization of UCP1 protein with insulin in human islets. UCP2 A55V was associated with waist circumference (P = 0.045) in AA, and BMI in SA (P = 0.018); and UCP2 G-866A with waist-to-hip ratio in AA (P = 0.016). Conclusion: This study suggests a functional variant of UCP1 contributes to the variance of AIRg in an AA population; the plausibility of this unexpected association is supported by the novel finding that UCP1 is expressed in islets. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi://10.1186/1472-6823-7-1 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852562/pdf/ en_US
dash.license LAA
dc.title The Uncoupling Protein 1 Gene, UCP1, is Expressed in Mammalian Islet Cells and Associated with Acute Insulin Response to Glucose in African American Families from the IRAS Family Study en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Endocrine Disorders en_US
dash.depositing.author Sharma, Arun J.
dc.date.available 2011-04-16T01:58:07Z
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US

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