dc.contributor.author | Putheti, Prabhakar | |
dc.contributor.author | Awasthi, Amit | |
dc.contributor.author | Popoola, Joyce | |
dc.contributor.author | Gao, Wenda | |
dc.contributor.author | Strom, Terry Barton | |
dc.date.accessioned | 2011-04-18T02:24:10Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Putheti, Prabhakar, Amit Awasthi, Joyce Popoola, Wenda Gao, and Terry B. Strom. 2010. Human CD4+ memory T cells can become CD4+IL-9+ T cells. PLoS ONE 5(1): e8706. | en_US |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4853406 | |
dc.description.abstract | Background: IL-9 is a growth factor for T- and mast-cells that is secreted by human Th2 cells. We recently reported that IL-4+TGF-β directs mouse CD4+CD25−CD62L+ T cells to commit to inflammatory IL-9 producing CD4+ T cells. Methodology/Principal Findings: Here we show that human inducible regulatory T cells (iTregs) also express IL-9. IL-4+TGF-β induced higher levels of IL-9 expression in plate bound-anti-CD3 mAb (pbCD3)/soluble-anti-CD28 mAb (sCD28) activated human resting memory CD4+CD25−CD45RO+ T cells as compared to naïve CD4+CD25−CD45RA+ T cells. In addition, as compared to pbCD3/sCD28 plus TGF-β stimulation, IL-4+TGF-β stimulated memory CD4+CD25−CD45RO+ T cells expressed reduced FOXP3 protein. As analyzed by pre-amplification boosted single-cell real-time PCR, human CD4+IL-9+ T cells expressed GATA3 and RORC, but not IL-10, IL-13, IFNγ or IL-17A/F. Attempts to optimize IL-9 production by pbCD3/sCD28 and IL-4+TGF-β stimulated resting memory CD4+ T cells demonstrated that the addition of IL-1β, IL-12, and IL-21 further enhance IL-9 production. Conclusions/Significance: Taken together these data show both the differences and similarities between mouse and human CD4+IL9+ T cells and reaffirm the powerful influence of inflammatory cytokines to shape the response of activated CD4+ T cells to antigen. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.isversionof | doi:10.1371/journal.pone.0008706 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806834/pdf/ | en_US |
dash.license | LAA | |
dc.title | Human CD4+ Memory T Cells Can Become CD4+IL-9+ T Cells | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | PLoS ONE | en_US |
dash.depositing.author | Putheti, Prabhakar | |
dc.date.available | 2011-04-18T02:24:10Z | |
dash.affiliation.other | HMS^Medicine- Beth Israel-Deaconess | en_US |
dash.affiliation.other | HMS^Neurology-Brigham and Women's Hospital | en_US |
dash.affiliation.other | HMS^Medicine- Beth Israel-Deaconess | en_US |
dash.affiliation.other | HMS^Surgery- Beth Israel-Deaconess | en_US |
dash.affiliation.other | HMS^Medicine- Beth Israel-Deaconess | en_US |
dc.identifier.doi | 10.1371/journal.pone.0008706 | * |
dash.contributor.affiliated | Awasthi, Amit | |
dash.contributor.affiliated | Putheti, P | |
dash.contributor.affiliated | Gao, W | |
dash.contributor.affiliated | Strom, Terry B. | |