Expression differences by continent of origin point to the immortalization process

DSpace/Manakin Repository

Expression differences by continent of origin point to the immortalization process

Citable link to this page

. . . . . .

Title: Expression differences by continent of origin point to the immortalization process
Author: Davis, Adam Robert; Kohane, Isaac Samuel

Note: Order does not necessarily reflect citation order of authors.

Citation: Davis, Adam R. and Isaac S. Kohane. 2009. Expression differences by continent of origin point to the immortalization process. Human Molecular Genetics 18(20): 3864-3875.
Full Text & Related Files:
Abstract: Analysis of recently available microarray expression data sets obtained from immortalized cell lines of the individuals represented in the HapMap project have led to inconclusive comparisons across cohorts with different ancestral continent of origin (ACOO). To address this apparent inconsistency, we applied a novel approach to accentuate population-specific gene expression signatures for the CEU [homogeneous US residents with northern and western European ancestry (HapMap samples)] and YRI [homogenous Yoruba people of Ibadan, Nigeria (HapMap samples)] trios. In this report, we describe how four independent data sets point to the differential expression across ACOO of gene networks implicated in transforming the normal lymphoblast into immortalized lymphoblastoid cells. In particular, Werner syndrome helicase and related genes are differentially expressed between the YRI and CEU cohorts. We further demonstrate that these differences correlate with viral titer and that both the titer and expression differences are associated with ACOO. We use the 14 genes most differentially expressed to construct an ACOO-specific ‘immortalization network’ comprised of 40 genes, one of which show significant correlation with genomic variation (eQTL). The extent to which these measured group differences are due to differences in the immortalization procedures used for each group or reflect ACOO-specific biological differences remains to be determined. That the ACOO group differences in gene expression patterns may depend strongly on the process of transforming cells to establish immortalized lines should be considered in such comparisons.
Published Version: doi:10.1093/hmg/ddp330
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748894/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4853414

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters