Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates.

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Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates.

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dc.contributor.author Mukherjee, Sutapa
dc.contributor.author Ngo, Long H.
dc.contributor.author Christiani, David C.
dc.contributor.author Kim, Jee Young
dc.date.accessioned 2011-04-19T02:44:23Z
dc.date.issued 2004
dc.identifier.citation Kim, Jee Young, Sutapa Mukherjee, Long C. Ngo, and David C. Christiani. 2004. Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates. Environmental Health Perspectives 112(6): 666-671. en_US
dc.identifier.issn 0091-6765 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4859257
dc.description.abstract Residual oil fly ash (ROFA) is a chemically complex mixture of compounds, including metals that are potentially carcinogenic because of their ability to cause oxidative injury. In this study, we investigated the association between exposure to particulate matter with an aerodynamic mass median diameter ≤ 2.5 micro m (PM2.5) and oxidative DNA damage and repair, as indicated by urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, in a group of boilermakers exposed to ROFA and metal fumes. Twenty workers (50% smokers) were monitored for 5 days during an overhaul of oil-fired boilers. The median occupational PM2.5 8-hr time-weighted average was 0.44 mg/m3 (25th-75th percentile, 0.29-0.76). The mean ± SE creatinine-adjusted 8-OHdG levels were 13.26 ± 1.04 micro μg/g in urine samples collected pre-workshift and 15.22 ± 0.99 micro μg/g in the post-workshift samples. The urinary 8-OHdG levels were significantly greater in the post-workshift samples than in the pre-workshift samples (p = 0.02), after adjusting for urinary cotinine levels, chronic bronchitis status, and age. Linear mixed models indicated a significant exposure-response association between PM2.5 exposure and urinary 8-OHdG levels (p = 0.03). Each 1-mg/m3 incremental increase in PM2.5 exposure was associated with an increase of 1.67 micro μg/g (95% confidence interval, 0.21-3.14) in 8-OHdG levels. PM2.5 vanadium, manganese, nickel, and lead exposures also were positively associated with 8-OHdG levels (p ≤ 0.05). This study suggests that a relatively young and healthy cohort of boilermakers may experience an increased risk of developing oxidative DNA injury after exposure to high levels of metal-containing particulate matter. en_US
dc.language.iso en_US en_US
dc.publisher National Institute of Environmental Health Sciences en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1241959/pdf/ en_US
dash.license LAA
dc.subject biomarkers en_US
dc.subject epidemiology en_US
dc.subject occupational en_US
dc.subject oxidative DNA damage en_US
dc.subject particulate matter en_US
dc.title Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates. en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Environmental Health Perspectives en_US
dash.depositing.author Christiani, David C.
dc.date.available 2011-04-19T02:44:23Z
dash.affiliation.other HMS^Medicine-Massachusetts General Hospital en_US
dash.affiliation.other SPH^Environmental+Occupational Medicine+Epi en_US

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