MicroSAGE is Highly Representative and Reproducible but Reveals Major Differences in Gene Expression Among Samples Obtained from Similar Tissues
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MicroSAGE is Highly Representative and Reproducible but Reveals Major Differences in Gene Expression Among Samples Obtained from Similar Tissues
| Title: | MicroSAGE is Highly Representative and Reproducible but Reveals Major Differences in Gene Expression Among Samples Obtained from Similar Tissues |
| Author: |
Blackshaw, Seth; Tsujikawa, Motokazu; Gunnersen, Jenny M; Scott, Hamish S; Boon, Wee-Ming; Tan, Seong-Seng; Kuo, Winston Patrick; Park, Peter J.; Cepko, Constance L.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Blackshaw, Seth, Winston P. Kuo, Peter J. Park, Motokazu Tsujikawa, Jenny M. Gunnersen, Hamish S. Scott, Wee-Ming Boon, Seong-Seng Tan, and Constance L. Cepko. 2003. MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues. Genome Biology 4(3): R17. |
| Full Text & Related Files: |
153457.pdf (528.6Kb; PDF) 
|
| Abstract: | Background: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. Results: We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals. Conclusions: MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently. |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC153457/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4870973 |
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