A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor

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A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor

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Title: A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor
Author: Deng, Lynn L.; Hett, Erik Christian; Chao, Michael; Rubin, Eric Joseph

Note: Order does not necessarily reflect citation order of authors.

Citation: Hett, Erik C., Michael C. Chao, Lynn L. Deng, and Eric J. Rubin. 2008. A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor. PLoS Pathogens 4(2): e1000001.
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Abstract: The final stage of bacterial cell division requires the activity of one or more enzymes capable of degrading the layers of peptidoglycan connecting two recently developed daughter cells. Although this is a key step in cell division and is required by all peptidoglycan-containing bacteria, little is known about how these potentially lethal enzymes are regulated. It is likely that regulation is mediated, at least partly, through protein–protein interactions. Two lytic transglycosylases of mycobacteria, known as resuscitation-promoting factor B and E (RpfB and RpfE), have previously been shown to interact with the peptidoglycan-hydrolyzing endopeptidase, Rpf-interacting protein A (RipA). These proteins may form a complex at the septum of dividing bacteria. To investigate the function of this potential complex, we generated depletion strains in M. smegmatis. Here we show that, while depletion of rpfB has no effect on viability or morphology, ripA depletion results in a marked decrease in growth and formation of long, branched chains. These growth and morphological defects could be functionally complemented by the M. tuberculosis ripA orthologue (rv1477), but not by another ripA-like orthologue (rv1478). Depletion of ripA also resulted in increased susceptibility to the cell wall–targeting β-lactams. Furthermore, we demonstrate that RipA has hydrolytic activity towards several cell wall substrates and synergizes with RpfB. These data reveal the unusual essentiality of a peptidoglycan hydrolase and suggest a novel protein–protein interaction as one way of regulating its activity.
Published Version: doi:10.1371/journal.ppat.1000001
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262848/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874698

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