A Transient Transgenic RNAi Strategy for Rapid Characterization of Gene Function during Embryonic Development

DSpace/Manakin Repository

A Transient Transgenic RNAi Strategy for Rapid Characterization of Gene Function during Embryonic Development

Citable link to this page

. . . . . .

Title: A Transient Transgenic RNAi Strategy for Rapid Characterization of Gene Function during Embryonic Development
Author: Davis, Shannon W.; Qiu, Haiyan; Saunders, Thomas L.; Sandy, Peter; Camper, Sally A.; Bjork, Bryan Cary; Fujiwara, Yuko; Orkin, Stuart Holland; Beier, David Randolph

Note: Order does not necessarily reflect citation order of authors.

Citation: Bjork, Bryan C., Yuko Fujiwara, Shannon W. Davis, Haiyan Qiu, Thomas L. Saunders, Peter Sandy, Stuart Orkin, Sally A. Camper, and David R. Beier. 2010. A transient transgenic RNAi strategy for rapid characterization of gene function during embryonic development. PLoS ONE 5(12): e14375.
Full Text & Related Files:
Abstract: RNA interference (RNAi) is a powerful strategy for studying the phenotypic consequences of reduced gene expression levels in model systems. To develop a method for the rapid characterization of the developmental consequences of gene dysregulation, we tested the use of RNAi for “transient transgenic” knockdown of mRNA in mouse embryos. These methods included lentiviral infection as well as transposition using the Sleeping Beauty (SB) and PiggyBac (PB) transposable element systems. This approach can be useful for phenotypic validation of putative mutant loci, as we demonstrate by confirming that knockdown of Prdm16 phenocopies the ENU-induced cleft palate (CP) mutant, csp1. This strategy is attractive as an alternative to gene targeting in embryonic stem cells, as it is simple and yields phenotypic information in a matter of weeks. Of the three methodologies tested, the PB transposon system produced high numbers of transgenic embryos with the expected phenotype, demonstrating its utility as a screening method.
Published Version: doi:10.1371/journal.pone.0014375
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002952/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874766

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters