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dc.contributor.authorYoung-Pearse, Tracy L
dc.contributor.authorChen, Allen Ching-Way
dc.contributor.authorChang, Rui
dc.contributor.authorMarquez, Cesar
dc.contributor.authorSelkoe, Dennis J.
dc.date.accessioned2011-04-23T01:03:01Z
dc.date.issued2008
dc.identifier.citationYoung-Pearse, Tracy L., Allen C. Chen, Rui Chang, Cesar Marquez, and Dennis J. Selkoe. 2008. Secreted APP regulates the function of full-length APP in neurite outgrowth through interaction with integrin beta1. Neural Development 3: 15.en_US
dc.identifier.issn1749-8104en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4874767
dc.description.abstractBackground: β-Amyloid precursor protein (APP) has been reported to play a role in the outgrowth of neurites from cultured neurons. Both cell-surface APP and its soluble, ectodomain cleavage product (APPs-α) have been implicated in regulating the length and branching of neurites in a variety of assays, but the mechanism by which APP performs this function is not understood. Results: Here, we report that APP is required for proper neurite outgrowth in a cell autonomous manner, both in vitro and in vivo. Neurons that lack APP undergo elongation of their longest neurite. Deletion of APLP1 or APLP2, homologues of APP, likewise stimulates neurite lengthening. Intriguingly, wild-type neurons exposed to APPs-α, the principal cleavage product of APP, also undergo neurite elongation. However, APPs-α is unable to stimulate neurite elongation in the absence of cellular APP expression. The outgrowth-enhancing effects of both APPs-α and the deletion of APP are inhibited by blocking antibodies to Integrin β1 (Itgβ1). Moreover, full length APP interacts biochemically with Itgβ1, and APPs-α can interfere with this binding. Conclusion: Our findings indicate that APPs-α regulates the function of APP in neurite outgrowth via the novel mechanism of competing with the binding of APP to Itgβ1.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1749-8104-3-15en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442059/pdf/en_US
dash.licenseLAA
dc.titleSecreted APP Regulates the Function of Full-Length APP in Neurite Outgrowth Through Interaction With Integrin Beta1en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNeural Developmenten_US
dash.depositing.authorSelkoe, Dennis J.
dc.date.available2011-04-23T01:03:01Z
dash.affiliation.otherHMS^Neurology-Brigham and Women's Hospitalen_US
dc.identifier.doi10.1186/1749-8104-3-15*
dash.contributor.affiliatedSelkoe, Dennis
dash.contributor.affiliatedChen, Allen


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