dc.contributor.author | Mattiazzi, M. | |
dc.contributor.author | Jambhekar, Ashwini | |
dc.contributor.author | Kaferle, P. | |
dc.contributor.author | DeRisi, J. L. | |
dc.contributor.author | Križaj, I. | |
dc.contributor.author | Petrovič, U. | |
dc.date.accessioned | 2011-04-23T18:50:44Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Mattiazzi, M., A. Jambhekar, P. Kaferle, J. L. DeRisi, I. Križaj, and U. Petrovič. 2010. Genetic interactions between a phospholipase A2 and the Rim101 pathway components in S. cerevisiae reveal a role for this pathway in response to changes in membrane composition and shape. Molecular Genetics and Genomics 283(6): 519-530. | en_US |
dc.identifier.issn | 1617-4615 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874830 | |
dc.description.abstract | Modulating composition and shape of biological membranes is an emerging mode of regulation of cellular processes. We investigated the global effects that such perturbations have on a model eukaryotic cell. Phospholipases A[sub]2 (PLA[sub]2s), enzymes that cleave one fatty acid molecule from membrane phospholipids, exert their biological activities through affecting both membrane composition and shape. We have conducted a genome-wide analysis of cellular effects of a PLA[sub]2 in the yeast Saccharomyces cerevisiae as a model system. We demonstrate functional genetic and biochemical interactions between PLA[sub]2 activity and the Rim101 signaling pathway in S. cerevisiae. Our results suggest that the composition and/or the shape of the endosomal membrane affect the Rim101 pathway. We describe a genetically and functionally related network, consisting of components of the Rim101 pathway and the prefoldin, retromer and SWR1 complexes, and predict its functional relation to PLA[sub]2 activity in a model eukaryotic cell. This study provides a list of the players involved in the global response to changes in membrane composition and shape in a model eukaryotic cell, and further studies are needed to understand the precise molecular mechanisms connecting them.Electronic supplementary material The online version of this article (doi:10.1007/s00438-010-0533-8) contains supplementary material, which is available to authorized users. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer-Verlag | en_US |
dc.relation.isversionof | doi:10.1007/s00438-010-0533-8 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872012/pdf/ | en_US |
dash.license | LAA | |
dc.subject | Rim101 pathway | en_US |
dc.subject | Phospholipase A2 | en_US |
dc.subject | endosomal membrane | en_US |
dc.subject | genetic interactions | en_US |
dc.title | Genetic interactions between a phospholipase A2 and the Rim101 pathway components in S. cerevisiae reveal a role for this pathway in response to changes in membrane composition and shape | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Molecular Genetics and Genomics | en_US |
dash.depositing.author | Jambhekar, Ashwini | |
dc.date.available | 2011-04-23T18:50:44Z | |
dash.affiliation.other | HMS^Genetics | en_US |
dc.identifier.doi | 10.1007/s00438-010-0533-8 | * |
dash.contributor.affiliated | Jambhekar, Ashwini | |