Accuracy and Precision in Quantitative Fluorescence Microscopy

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Accuracy and Precision in Quantitative Fluorescence Microscopy

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dc.contributor.author Waters, Jennifer Catherine
dc.date.accessioned 2011-04-26T02:05:53Z
dc.date.issued 2009
dc.identifier.citation Waters, Jennifer C. 2009. Accuracy and precision in quantitative fluorescence microscopy. The Journal of Cell Biology 185(7): 1135-1148. en_US
dc.identifier.issn 0021-9525 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4875892
dc.description.abstract The light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the discovery and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens. Whether simply comparing the relative intensities of two fluorescent specimens, or using advanced techniques like Förster resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP), quantitation of fluorescence requires a thorough understanding of the limitations of and proper use of the different components of the imaging system. Here, I focus on the parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements. en_US
dc.language.iso en_US en_US
dc.publisher The Rockefeller University Press en_US
dc.relation.isversionof doi:10.1083/jcb.200903097 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712964/pdf/ en_US
dash.license LAA
dc.title Accuracy and Precision in Quantitative Fluorescence Microscopy en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal The Journal of Cell Biology en_US
dash.depositing.author Waters, Jennifer Catherine
dc.date.available 2011-04-26T02:05:53Z
dash.affiliation.other HMS^Cell Biology en_US

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