Conserved Genes Act as Modifiers of Invertebrate SMN Loss of Function Defects

DSpace/Manakin Repository

Conserved Genes Act as Modifiers of Invertebrate SMN Loss of Function Defects

Citable link to this page

. . . . . .

Title: Conserved Genes Act as Modifiers of Invertebrate SMN Loss of Function Defects
Author: Dimitriadi, Maria; Sleigh, James N.; Chang, Howard C.; Kalloo, Geetika; Harris, Jevede; Barsby, Tom; Walsh, Melissa B.; Satterlee, John S.; Li, Chris; Hart, Anne C.; Walker, Amy K.; Sen, Anindya Kumar; Van Vactor, David L.; Artavanis-Tsakonas, Spiro

Note: Order does not necessarily reflect citation order of authors.

Citation: Dimitriadi, Maria, James N. Sleigh, Amy Walker, Howard C. Chang, Anindya Sen, Geetika Kalloo, Jevede Harris, et al. 2010. Conserved genes act as modifiers of invertebrate SMN loss of function defects. PLoS Genetics 6(10): e1001172.
Full Text & Related Files:
Abstract: Spinal Muscular Atrophy (SMA) is caused by diminished function of the Survival of Motor Neuron (SMN) protein, but the molecular pathways critical for SMA pathology remain elusive. We have used genetic approaches in invertebrate models to identify conserved SMN loss of function modifier genes. Drosophila melanogaster and Caenorhabditis elegans each have a single gene encoding a protein orthologous to human SMN; diminished function of these invertebrate genes causes lethality and neuromuscular defects. To find genes that modulate SMN function defects across species, two approaches were used. First, a genome-wide RNAi screen for C. elegans SMN modifier genes was undertaken, yielding four genes. Second, we tested the conservation of modifier gene function across species; genes identified in one invertebrate model were tested for function in the other invertebrate model. Drosophila orthologs of two genes, which were identified originally in C. elegans, modified Drosophila SMN loss of function defects. C. elegans orthologs of twelve genes, which were originally identified in a previous Drosophila screen, modified C. elegans SMN loss of function defects. Bioinformatic analysis of the conserved, cross-species, modifier genes suggests that conserved cellular pathways, specifically endocytosis and mRNA regulation, act as critical genetic modifiers of SMN loss of function defects across species.
Published Version: doi:10.1371/journal.pgen.1001172
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965752/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4875899

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters