Sarcomere Formation Occurs by the Assembly of Multiple Latent Protein Complexes

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Sarcomere Formation Occurs by the Assembly of Multiple Latent Protein Complexes

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dc.contributor.author Rui, Yanning
dc.contributor.author Bai, Jianwu
dc.contributor.author Perrimon, Norbert
dc.date.accessioned 2011-04-27T00:43:16Z
dc.date.issued 2010
dc.identifier.citation Rui, Yanning, Jianwu Bai, and Norbert Perrimon. 2010. Sarcomere formation occurs by the assembly of multiple latent protein complexes. PLoS Genetics 6(11): e1001208. en_US
dc.identifier.issn 1553-7390 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878056
dc.description.abstract The stereotyped striation of myofibrils is a conserved feature of muscle organization that is critical to its function. Although most components that constitute the basic myofibrils are well-characterized biochemically and are conserved across the animal kingdom, the mechanisms leading to the precise assembly of sarcomeres, the basic units of myofibrils, are poorly understood. To gain insights into this process, we investigated the functional relationships of sarcomeric protein complexes. Specifically, we systematically analyzed, using either RNAi in primary muscle cells or available genetic mutations, the organization of myofibrils in Drosophila muscles that lack one or more sarcomeric proteins. Our study reveals that the thin and thick filaments are mutually dependent on each other for striation. Further, the tension sensor complex comprised of zipper/Zasp/α-actinin is involved in stabilizing the sarcomere but not in its initial formation. Finally, integrins appear essential for the interdigitation of thin and thick filaments that occurs prior to striation. Thus, sarcomere formation occurs by the coordinated assembly of multiple latent protein complexes, as opposed to sequential assembly. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pgen.1001208 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987826/pdf/ en_US
dash.license LAA
dc.subject cell biology en_US
dc.subject developmental biology en_US
dc.subject genetics and genomics en_US
dc.subject gene function en_US
dc.title Sarcomere Formation Occurs by the Assembly of Multiple Latent Protein Complexes en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Genetics en_US
dash.depositing.author Perrimon, Norbert
dc.date.available 2011-04-27T00:43:16Z
dash.affiliation.other HMS^Genetics en_US

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