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dc.contributor.authorRui, Yanning
dc.contributor.authorBai, Jianwu
dc.contributor.authorPerrimon, Norbert
dc.date.accessioned2011-04-27T00:43:16Z
dc.date.issued2010
dc.identifier.citationRui, Yanning, Jianwu Bai, and Norbert Perrimon. 2010. Sarcomere formation occurs by the assembly of multiple latent protein complexes. PLoS Genetics 6(11): e1001208.en_US
dc.identifier.issn1553-7390en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4878056
dc.description.abstractThe stereotyped striation of myofibrils is a conserved feature of muscle organization that is critical to its function. Although most components that constitute the basic myofibrils are well-characterized biochemically and are conserved across the animal kingdom, the mechanisms leading to the precise assembly of sarcomeres, the basic units of myofibrils, are poorly understood. To gain insights into this process, we investigated the functional relationships of sarcomeric protein complexes. Specifically, we systematically analyzed, using either RNAi in primary muscle cells or available genetic mutations, the organization of myofibrils in Drosophila muscles that lack one or more sarcomeric proteins. Our study reveals that the thin and thick filaments are mutually dependent on each other for striation. Further, the tension sensor complex comprised of zipper/Zasp/α-actinin is involved in stabilizing the sarcomere but not in its initial formation. Finally, integrins appear essential for the interdigitation of thin and thick filaments that occurs prior to striation. Thus, sarcomere formation occurs by the coordinated assembly of multiple latent protein complexes, as opposed to sequential assembly.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pgen.1001208en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987826/pdf/en_US
dash.licenseLAA
dc.subjectcell biologyen_US
dc.subjectdevelopmental biologyen_US
dc.subjectgenetics and genomicsen_US
dc.subjectgene functionen_US
dc.titleSarcomere Formation Occurs by the Assembly of Multiple Latent Protein Complexesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Geneticsen_US
dash.depositing.authorPerrimon, Norbert
dc.date.available2011-04-27T00:43:16Z
dash.affiliation.otherHMS^Geneticsen_US
dc.identifier.doi10.1371/journal.pgen.1001208*
dash.contributor.affiliatedPerrimon, Norbert


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