Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus

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Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus

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dc.contributor.author Kuraguchi, Mari
dc.contributor.author Wang, Xiu-Ping
dc.contributor.author Rothenberg, Rebecca
dc.contributor.author Ohene-Baah, Nana Yaw
dc.contributor.author Lund, Jennifer J
dc.contributor.author Bronson, Roderick Terry
dc.contributor.author Kucherlapati, Melanie Haas
dc.contributor.author Maas, Richard Louis
dc.contributor.author Kucherlapati, Raju
dc.date.accessioned 2011-04-27T02:48:15Z
dc.date.issued 2006
dc.identifier.citation Kuraguchi, Mari, Xiu-Ping Wang, Roderick T. Bronson, Rebecca Rothenberg, Nana Yaw Ohene-Baah, Jennifer J. Lund, Melanie Kucherlapati, Richard L. Maas, and Raju Kucherlapati. 2006. Adenomatous Polyposis Coli (APC) is required for normal development of skin and thymus. PLoS Genetics 2(9): e146. en_US
dc.identifier.issn 1553-7390 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878073
dc.description.abstract The tumor suppressor gene Apc (adenomatous polyposis coli) is a member of the Wnt signaling pathway that is involved in development and tumorigenesis. Heterozygous knockout mice for Apc have a tumor predisposition phenotype and homozygosity leads to embryonic lethality. To understand the role of Apc in development we generated a floxed allele. These mice were mated with a strain carrying Cre recombinase under the control of the human Keratin 14 (K14) promoter, which is active in basal cells of epidermis and other stratified epithelia. Mice homozygous for the floxed allele that also carry the K14-cre transgene were viable but had stunted growth and died before weaning. Histological and immunochemical examinations revealed that K14-cre–mediated Apc loss resulted in aberrant growth in many ectodermally derived squamous epithelia, including hair follicles, teeth, and oral and corneal epithelia. In addition, squamous metaplasia was observed in various epithelial-derived tissues, including the thymus. The aberrant growth of hair follicles and other appendages as well as the thymic abnormalities in K14-cre; ApcCKO/CKO mice suggest the Apc gene is crucial in embryonic cells to specify epithelial cell fates in organs that require epithelial–mesenchymal interactions for their development. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pgen.0020146 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1564426/pdf/ en_US
dash.license LAA
dc.subject genetics en_US
dc.subject gene function en_US
dc.subject Mus (mouse) en_US
dc.title Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Genetics en_US
dash.depositing.author Kucherlapati, Raju
dc.date.available 2011-04-27T02:48:15Z
dash.affiliation.other HMS^Pathology en_US
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US
dash.affiliation.other HMS^Medicine-Brigham and Women's Hospital en_US
dash.affiliation.other HMS^Genetics en_US

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