Keratins Regulate Protein Biosynthesis Through Localization of GLUT1 and -3 Upstream of AMP Kinase and Raptor

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Keratins Regulate Protein Biosynthesis Through Localization of GLUT1 and -3 Upstream of AMP Kinase and Raptor

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Title: Keratins Regulate Protein Biosynthesis Through Localization of GLUT1 and -3 Upstream of AMP Kinase and Raptor
Author: Kröger, Cornelia; Reuter, Ursula; Windoffer, Reinhard; Leube, Rudolf E.; Magin, Thomas M.; Vijayaraj, Preethi

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Citation: Vijayaraj, Preethi, Cornelia Kröger, Ursula Reuter, Reinhard Windoffer, Rudolf E. Leube, and Thomas M. Magin. 2009. Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor. The Journal of Cell Biology 187(2): 175-184.
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Abstract: Keratin intermediate filament proteins form cytoskeletal scaffolds in epithelia, the disruption of which affects cytoarchitecture, cell growth, survival, and organelle transport. However, owing to redundancy, the global function of keratins has not been defined in full. Using a targeted gene deletion strategy, we generated transgenic mice lacking the entire keratin multiprotein family. In this study, we report that without keratins, embryonic epithelia suffer no cytolysis and maintain apical polarity but display mislocalized desmosomes. All keratin­null embryos die from severe growth retardation at embryonic day 9.5. We find that GLUT1 and ­3 are mislocalized from the apical plasma membrane in embryonic epithelia, which subsequently activates the energy sensor adenosine monophosphate kinase (AMPK). Analysis of the mammalian target of rapamycin (mTOR) pathway reveals that AMPK induction activates Raptor, repressing protein biosynthesis through mTORC1’s downstream targets S6 kinase and 4E­binding protein 1. Our findings demonstrate a novel keratin function upstream of mTOR signaling via GLUT localization and have implications for pathomechanisms and therapy approaches for keratin disorders and the analysis of other gene families.
Published Version: doi:10.1083/jcb.200906094
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768834/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878079

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