Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate
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Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate
| Title: | Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate |
| Author: |
Inan, Mehmet; Festjens, Nele; Haustraete, Jurgen; Van Hecke, Annelies; Contreras, Roland; Meagher, Michael M; Callewaert, Nico; Jacobs, Pieter P.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Jacobs, Pieter P., Mehmet Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, Michael M. Meagher, and Nico Callewaert. 2010. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9: 93. |
| Full Text & Related Files: |
3004841.pdf (1.201Mb; PDF) 
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| Abstract: | Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins. |
| Published Version: | doi:10.1186/1475-2859-9-93 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004841/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878941 |
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