Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate
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Inan, Mehmet
Festjens, Nele
Haustraete, Jurgen
Van Hecke, Annelies
Contreras, Roland
Meagher, Michael M
Callewaert, Nico
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https://doi.org/10.1186/1475-2859-9-93Metadata
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Jacobs, Pieter P., Mehmet Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, Michael M. Meagher, and Nico Callewaert. 2010. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9: 93.Abstract
Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004841/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878941
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