Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

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Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

dc.contributor.author	Inan, Mehmet
dc.contributor.author	Festjens, Nele
dc.contributor.author	Haustraete, Jurgen
dc.contributor.author	Van Hecke, Annelies
dc.contributor.author	Contreras, Roland
dc.contributor.author	Meagher, Michael M
dc.contributor.author	Callewaert, Nico
dc.contributor.author	Jacobs, Pieter P.
dc.date.accessioned	2011-04-28T06:26:16Z
dc.date.issued	2010
dc.identifier.citation	Jacobs, Pieter P., Mehmet Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, Michael M. Meagher, and Nico Callewaert. 2010. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9: 93.	en_US
dc.identifier.issn	1475-2859	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878941
dc.description.abstract	Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi:10.1186/1475-2859-9-93	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004841/pdf/	en_US
dash.license	LAA
dc.title	Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Microbial Cell Factories	en_US
dash.depositing.author	Jacobs, Pieter P.
dc.date.available	2011-04-28T06:26:16Z
dash.affiliation.other	HMS^Dermatology-Brigham and Women's Hospital	en_US