Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

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Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

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dc.contributor.author Inan, Mehmet
dc.contributor.author Festjens, Nele
dc.contributor.author Haustraete, Jurgen
dc.contributor.author Van Hecke, Annelies
dc.contributor.author Contreras, Roland
dc.contributor.author Meagher, Michael M
dc.contributor.author Callewaert, Nico
dc.contributor.author Jacobs, Pieter P.
dc.date.accessioned 2011-04-28T06:26:16Z
dc.date.issued 2010
dc.identifier.citation Jacobs, Pieter P., Mehmet Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, Michael M. Meagher, and Nico Callewaert. 2010. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9: 93. en_US
dc.identifier.issn 1475-2859 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878941
dc.description.abstract Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1475-2859-9-93 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004841/pdf/ en_US
dash.license LAA
dc.title Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Microbial Cell Factories en_US
dash.depositing.author Jacobs, Pieter P.
dc.date.available 2011-04-28T06:26:16Z
dash.affiliation.other HMS^Dermatology-Brigham and Women's Hospital en_US

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