GEF-H1 Mediated Control of NOD1 Dependent NF-κB Activation by Shigella Effectors

DSpace/Manakin Repository

GEF-H1 Mediated Control of NOD1 Dependent NF-κB Activation by Shigella Effectors

Show simple item record

dc.contributor.author Fukazawa, Atsuko
dc.contributor.author Alonso, Carmen
dc.contributor.author Kurachi, Kiyotaka
dc.contributor.author Gupta, Sonal
dc.contributor.author Philpott, Dana J.
dc.contributor.author Lesser, Cammie
dc.contributor.author McCormick, Beth A.
dc.contributor.author Reinecker, Hans-Christian
dc.date.accessioned 2011-04-29T01:45:18Z
dc.date.issued 2008
dc.identifier.citation Fukazawa, Atsuko, Carmen Alonso, Kiyotaka Kurachi, Sonal Gupta, Cammie F. Lesser, Beth Ann McCormick, and Hans-Christian Reinecker. 2008. GEF-H1 Mediated Control of NOD1 Dependent NF-κB Activation by Shigella Effectors. PLoS Pathogens 4(11): e1000228. en_US
dc.identifier.issn 1553-7366 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4879197
dc.description.abstract Shigella flexneri has evolved the ability to modify host cell function with intracellular active effectors to overcome the intestinal barrier. The detection of these microbial effectors and the initiation of innate immune responses are critical for rapid mucosal defense activation. The guanine nucleotide exchange factor H1 (GEF-H1) mediates RhoA activation required for cell invasion by the enteroinvasive pathogen Shigella flexneri. Surprisingly, GEF-H1 is requisite for NF-κB activation in response to Shigella infection. GEF-H1 interacts with NOD1 and is required for RIP2 dependent NF-κB activation by H-Ala-D-γGlu-DAP (γTriDAP). GEF-H1 is essential for NF-κB activation by the Shigella effectors IpgB2 and OspB, which were found to signal in a NOD1 and RhoA Kinase (ROCK) dependent manner. Our results demonstrate that GEF-H1 is a critical component of cellular defenses forming an intracellular sensing system with NOD1 for the detection of microbial effectors during cell invasion by pathogens. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.ppat.1000228 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583055/pdf/ en_US
dash.license LAA
dc.subject cell biology en_US
dc.subject cell signaling en_US
dc.subject cytoskeleton en_US
dc.subject gastroenterology and hepatology en_US
dc.subject gastrointestinal infections en_US
dc.subject immunology en_US
dc.subject innate immunity en_US
dc.subject microbiology en_US
dc.title GEF-H1 Mediated Control of NOD1 Dependent NF-κB Activation by Shigella Effectors en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS Pathogens en_US
dash.depositing.author Lesser, Cammie
dc.date.available 2011-04-29T01:45:18Z
dash.affiliation.other HMS^Medicine-Massachusetts General Hospital en_US
dash.affiliation.other HMS^Pediatrics-Massachusetts General Hospital en_US
dash.affiliation.other HMS^Medicine-Massachusetts General Hospital en_US

Files in this item

Files Size Format View
2583055.pdf 2.947Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters