Large-Scale Mapping of Mutations Affecting Zebrafish Development

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Large-Scale Mapping of Mutations Affecting Zebrafish Development

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dc.contributor.author Geisler, Robert
dc.contributor.author Rauch, Gerd-Jörg
dc.contributor.author Geiger-Rudolph, Silke
dc.contributor.author Albrecht, Andrea
dc.contributor.author van Bebber, Frauke
dc.contributor.author Berger, Andrea
dc.contributor.author Busch-Nentwich, Elisabeth
dc.contributor.author Dahm, Ralf
dc.contributor.author Dekens, Marcus PS
dc.contributor.author Dooley, Christopher
dc.contributor.author Elli, Alexandra F
dc.contributor.author Gehring, Ines
dc.contributor.author Geiger, Horst
dc.contributor.author Geisler, Maria
dc.contributor.author Glaser, Stefanie
dc.contributor.author Holley, Scott
dc.contributor.author Huber, Matthias
dc.contributor.author Kerr, Andy
dc.contributor.author Kirn, Anette
dc.contributor.author Knirsch, Martina
dc.contributor.author Konantz, Martina
dc.contributor.author Küchler, Axel M
dc.contributor.author Maderspacher, Florian
dc.contributor.author Neuhauss, Stephan C
dc.contributor.author Nicolson, Teresa
dc.contributor.author Ober, Elke A
dc.contributor.author Rentzsch, Brit
dc.contributor.author Rick, Jens M
dc.contributor.author Rief, Eva
dc.contributor.author Schauerte, Heike E
dc.contributor.author Schepp, Carsten P
dc.contributor.author Schönberger, Ulrike
dc.contributor.author Schonthaler, Helia B
dc.contributor.author Seiler, Christoph
dc.contributor.author Sidi, Samuel
dc.contributor.author Söllner, Christian
dc.contributor.author Wehner, Anja
dc.contributor.author Weiler, Christian
dc.contributor.author Nüsslein-Volhard, Christiane
dc.contributor.author Praeg, Elke
dc.contributor.author Ray, Russell
dc.date.accessioned 2011-05-03T15:59:27Z
dc.date.issued 2007
dc.identifier.citation Geisler, Robert, Gerd-Jörg Rauch, Silke Geiger-Rudolph, Andrea Albrecht, Frauke van Bebber, Andrea Berger, Elisabeth Busch-Nentwich, et al. 2007. Large-scale mapping of mutations affecting zebrafish development. BMC Genomics 8: 11. en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4881144
dc.description.abstract Background: Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers. Results: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM. Conclusion: By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2164-8-11 en_US
dash.license LAA
dc.title Large-Scale Mapping of Mutations Affecting Zebrafish Development en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Genomics en_US
dash.depositing.author Praeg, Elke
dc.date.available 2011-05-03T15:59:27Z
dash.affiliation.other HMS^Neurology- Beth Israel-Deaconess en_US

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