Osteopetrotic (op/op) Mice Have Reduced Microglia, No Aβ Deposition, and No Changes in Dopaminergic Neurons
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Osteopetrotic (op/op) Mice Have Reduced Microglia, No Aβ Deposition, and No Changes in Dopaminergic Neurons
| Title: | Osteopetrotic (op/op) Mice Have Reduced Microglia, No Aβ Deposition, and No Changes in Dopaminergic Neurons |
| Author: |
Kondo, Yoichi; Seabrook, Timothy J; Lemere, Cynthia Ann
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Kondo, Yoichi, Cynthia A. Lemere, and Timothy J. Seabrook. 2007. Osteopetrotic (op/op) mice have reduced microglia, no Aβ deposition, and no changes in dopaminergic neurons. Journal of Neuroinflammation 4: 31. |
| Full Text & Related Files: |
2234402.pdf (1.148Mb; PDF) 
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| Abstract: | Background: Activation of microglia is a part of the inflammatory response in neurodegenerative diseases but its role in the pathophysiology of these diseases is still unclear. The osteopetrotic (op/op) mouse lacks colony-stimulating factor-1 (CSF-1) and thus has a deficiency in microglia and macrophages. Prior reports have demonstrated that op/op mice deposit amyloid β (Aβ) plaques, similar to those found in Alzheimer's disease. The purpose of these studies was to confirm this and to determine if the lack of CSF-1 affects the development of dopaminergic neurons and the expression of CD200, a known microglial inhibitory protein. Method: We examined the central nervous system of op/op mice at 30 days, 60 days and 7 months of age and wildtype littermates at 30 days using immunohistochemistry and histochemistry. Results: We found a decrease in the number of microglia in 1 month-old op/op mice compared to wildtype (WT) littermates as measured by CD11b, CD45, CD32/16, CD68, CD204 and F4/80 immunoreactivity. Aβ plaques were not detected, while the number of dopaminergic neurons appeared normal. The expression of CD200 appeared to be normal, but there appeared to be a lower expression in the substantia nigra. Conclusion: In contrast to a prior report we did not detect Aβ deposition in the central nervous system of op/op mice at 30 days, 60 days or 7 months of age and there was a normal number of dopaminergic neurons. This indicates that op/op mice may be useful to examine the effects of microglia on neurodegenerative disease progression by breeding them to different transgenic mouse models. In addition, the lack of CSF-1 does not appear to affect CD200 expression by neurons but we did note a decrease in the substantia nigra of op/op and WT mice, suggesting that this may be a mechanism by which microglia control may be attenuated in this specific area during Parkinson's disease. |
| Published Version: | doi:10.1186/1742-2094-4-31 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234402/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4882676 |
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