Identification of Novel Pro-Migratory, Cancer-Associated Genes Using Quantitative, Microscopy-Based Screening

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Identification of Novel Pro-Migratory, Cancer-Associated Genes Using Quantitative, Microscopy-Based Screening

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dc.contributor.author Naffar-Abu-Amara, Suha
dc.contributor.author Shay, Tal
dc.contributor.author Galun, Meirav
dc.contributor.author Cohen, Naomi
dc.contributor.author Kam, Zvi
dc.contributor.author Geiger, Benjamin
dc.contributor.author Isakoff, Steven Jay
dc.date.accessioned 2011-05-05T16:06:20Z
dc.date.issued 2008
dc.identifier.citation Naffar-Abu-Amara, Suha, Tal Shay, Meirav Galun, Naomi Cohen, Steven J. Isakoff, Zvi Kam, and Benjamin Geiger. 2008. Identification of novel pro-migratory, cancer-associated genes using quantitative, microscopy-based screening. PLoS ONE 3(1): e1457. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4882758
dc.description.abstract Background: Cell migration is a highly complex process, regulated by multiple genes, signaling pathways and external stimuli. To discover genes or pharmacological agents that can modulate the migratory activity of cells, screening strategies that enable the monitoring of diverse migratory parameters in a large number of samples are necessary. Methodology: In the present study, we describe the development of a quantitative, high-throughput cell migration assay, based on a modified phagokinetic tracks (PKT) procedure, and apply it for identifying novel pro-migratory genes in a cancer-related gene library. In brief, cells are seeded on fibronectin-coated 96-well plates, covered with a monolayer of carboxylated latex beads. Motile cells clear the beads, located along their migratory paths, forming tracks that are visualized using an automated, transmitted-light screening microscope. The tracks are then segmented and characterized by multi-parametric, morphometric analysis, resolving a variety of morphological and kinetic features. Conclusions: In this screen we identified 4 novel genes derived from breast carcinoma related cDNA library, whose over-expression induces major alteration in the migration of the stationary MCF7 cells. This approach can serve for high throughput screening for novel ways to modulate cellular migration in pathological states such as tumor metastasis and invasion. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0001457 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195451/pdf/ en_US
dash.license LAA
dc.subject cell biology en_US
dc.subject cell adhesion en_US
dc.subject cytoskeleton en_US
dc.subject gene expression en_US
dc.title Identification of Novel Pro-Migratory, Cancer-Associated Genes Using Quantitative, Microscopy-Based Screening en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Isakoff, Steven Jay
dc.date.available 2011-05-05T16:06:20Z
dash.affiliation.other HMS^Medicine-Massachusetts General Hospital en_US

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