Adaptive Autoimmunity and Foxp3-Based Immunoregulation in Zebrafish

DSpace/Manakin Repository

Adaptive Autoimmunity and Foxp3-Based Immunoregulation in Zebrafish

Show simple item record

dc.contributor.author Iglesias, Antonio H.
dc.contributor.author Farez, Mauricio F.
dc.contributor.author Caccamo, Mario
dc.contributor.author Burns, Evan J.
dc.contributor.author Kassam, Nasim
dc.contributor.author Quintana, Francisco Javier
dc.contributor.author Oukka, Mohamed
dc.contributor.author Weiner, Howard Lee
dc.date.accessioned 2011-05-06T17:45:59Z
dc.date.issued 2010
dc.identifier.citation Quintana, Francisco J., Antonio H. Iglesias, Mauricio F. Farez, Mario Caccamo, Evan J. Burns, Nasim Kassam, Mohamed Oukka, and Howard L. Weiner. 2010. Adaptive autoimmunity and Foxp3-based immunoregulation in zebrafish. PLoS ONE 5(3). en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4882825
dc.description.abstract Background: Jawed vertebrates generate their immune-receptor repertoire by a recombinatorial mechanism that has the potential to produce harmful autoreactive lymphocytes. In mammals, peripheral tolerance to self-antigens is enforced by Foxp3+ regulatory T cells. Recombinatorial mechanisms also operate in teleosts, but active immunoregulation is thought to be a late incorporation to the vertebrate lineage. Methods/Principal Findings: Here we report the characterization of adaptive autoimmunity and Foxp3-based immunoregulation in the zebrafish. We found that zebrafish immunization with an homogenate of zebrafish central nervous system (zCNS) triggered CNS inflammation and specific antibodies. We cloned the zebrafish ortholog for mammalian Foxp3 (zFoxp3) which induced a regulatory phenotype on mouse T cells and controlled IL-17 production in zebrafish embryos. Conclusions/Significance: Our findings demonstrate the acquisition of active mechanisms of self-tolerance early in vertebrate evolution, suggesting that active regulatory mechanisms accompany the development of the molecular potential for adaptive autoimmunity. Moreover, they identify the zebrafish as a tool to study the molecular pathways controlling adaptive immunity. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi://10.1371/journal.pone.0009478 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832694/pdf/ en_US
dash.license LAA
dc.subject immunology en_US
dc.subject autoimmunity en_US
dc.subject immune response en_US
dc.subject immunomodulation en_US
dc.title Adaptive Autoimmunity and Foxp3-Based Immunoregulation in Zebrafish en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Weiner, Howard Lee
dc.date.available 2011-05-06T17:45:59Z
dash.affiliation.other HMS^Neurology-Brigham and Women's Hospital en_US
dash.affiliation.other HMS^Neurology-Brigham and Women's Hospital en_US

Files in this item

Files Size Format View
2832694.pdf 1.461Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters