Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells

DSpace/Manakin Repository

Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells

Citable link to this page

 

 
Title: Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells
Author: Brodsky, Alexander S; Hall, Giles; Keenan, Benjamin J; Meyer, Clifford; Swinburne, Ian A; Liu, Xiaole Shirley; Fox, Edward Alvin; Silver, Pamela A.

Note: Order does not necessarily reflect citation order of authors.

Citation: Brodsky, Alexander S., Clifford A. Meyer, Ian A. Swinburne, Giles Hall, Benjamin J. Keenan, Xiaole S. Liu, Edward A. Fox, and Pamela A. Silver. 2005. Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biology 6(8): R64.
Full Text & Related Files:
Abstract: Background: Transcription by RNA polymerase II is regulated at many steps including initiation,
promoter release, elongation and termination. Accumulation of RNA polymerase II at particular
locations across genes can be indicative of sites of regulation. RNA polymerase II is thought to
accumulate at the promoter and at sites of co-transcriptional alternative splicing where the rate of
RNA synthesis slows.
Results: To further understand transcriptional regulation at a global level, we determined the
distribution of RNA polymerase II within regions of the human genome designated by the
ENCODE project. Hypophosphorylated RNA polymerase II localizes almost exclusively to 5' ends
of genes. On the other hand, localization of total RNA polymerase II reveals a variety of distinct
landscapes across many genes with 74% of the observed enriched locations at exons. RNA
polymerase II accumulates at many annotated constitutively spliced exons, but is biased for
alternatively spliced exons. Finally, RNA polymerase II is also observed at locations not in gene
regions.
Conclusion: Localizing RNA polymerase II across many millions of base pairs in the human
genome identifies novel sites of transcription and provides insights into the regulation of
transcription elongation. These data indicate that RNA polymerase II accumulates most often at
exons during transcription. Thus, a major factor of transcription elongation control in mammalian
cells is the coordination of transcription and pre-mRNA processing to define exons.
Published Version: doi:10.1186/gb-2005-6-8-r64
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1273631/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4887112
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters