Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells

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Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells

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Title: Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells
Author: Brodsky, Alexander S; Hall, Giles; Keenan, Benjamin J; Meyer, Clifford; Swinburne, Ian A; Liu, Xiaole Shirley; Fox, Edward Alvin; Silver, Pamela A.

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Citation: Brodsky, Alexander S., Clifford A. Meyer, Ian A. Swinburne, Giles Hall, Benjamin J. Keenan, Xiaole S. Liu, Edward A. Fox, and Pamela A. Silver. 2005. Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biology 6(8): R64.
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Abstract: Background: Transcription by RNA polymerase II is regulated at many steps including initiation, promoter release, elongation and termination. Accumulation of RNA polymerase II at particular locations across genes can be indicative of sites of regulation. RNA polymerase II is thought to accumulate at the promoter and at sites of co-transcriptional alternative splicing where the rate of RNA synthesis slows. Results: To further understand transcriptional regulation at a global level, we determined the distribution of RNA polymerase II within regions of the human genome designated by the ENCODE project. Hypophosphorylated RNA polymerase II localizes almost exclusively to 5' ends of genes. On the other hand, localization of total RNA polymerase II reveals a variety of distinct landscapes across many genes with 74% of the observed enriched locations at exons. RNA polymerase II accumulates at many annotated constitutively spliced exons, but is biased for alternatively spliced exons. Finally, RNA polymerase II is also observed at locations not in gene regions. Conclusion: Localizing RNA polymerase II across many millions of base pairs in the human genome identifies novel sites of transcription and provides insights into the regulation of transcription elongation. These data indicate that RNA polymerase II accumulates most often at exons during transcription. Thus, a major factor of transcription elongation control in mammalian cells is the coordination of transcription and pre-mRNA processing to define exons.
Published Version: doi:10.1186/gb-2005-6-8-r64
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1273631/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4887112

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