Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines

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Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines

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dc.contributor.author Gupta, Sachin
dc.contributor.author Termini, James M
dc.contributor.author Niu, Liguo
dc.contributor.author Kanagavelu, Saravana K
dc.contributor.author Kornbluth, Richard S
dc.contributor.author Stone, Geoffrey W
dc.contributor.author Evans, David T.
dc.contributor.author Rahmberg, Andrew Richard
dc.date.accessioned 2011-09-13T17:46:27Z
dc.date.issued 2011
dc.identifier.citation Gupta, Sachin, James M. Termini, Liguo Niu, Saravana K. Kanagavelu, Andrew R. Rahmberg, Richard S. Kornbluth, David T. Evans, and Geoffrey W. Stone. 2011. Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. Retrovirology 8:39. en_US
dc.identifier.issn 1742-4690 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5130450
dc.description.abstract Background Molecular adjuvants are a promising method to enhance virus-specific immune responses and protect against HIV-1 infection. Immune activation by ligands for receptors such as CD40 can induce dendritic cell activation and maturation. Here we explore the incorporation of two CD40 mimics, Epstein Barr Virus gene LMP1 or an LMP1-CD40 chimera, into a strain of SIV that was engineered to be limited to a single cycle of infection. Results Full length LMP1 or the chimeric protein LMP1-CD40 was cloned into the nef-locus of single-cycle SIV. Human and Macaque monocyte derived macrophages and DC were infected with these viruses. Infected cells were analyzed for activation surface markers by flow cytometry. Cells were also analyzed for secretion of pro-inflammatory cytokines IL-1β, IL-6, IL-8, IL-12p70 and TNF by cytometric bead array. Conclusions Overall, single-cycle SIV expressing LMP1 and LMP1-CD40 produced a broad and potent T\(_H\)1-biased immune response in human as well as rhesus macaque macrophages and DC when compared with control virus. Single-cycle SIV-LMP1 also enhanced antigen presentation by lentiviral vector vaccines, suggesting that LMP1-mediated immune activation may enhance lentiviral vector vaccines against HIV-1. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi://10.1186/1742-4690-8-39 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118346/pdf/ en_US
dash.license LAA
dc.title Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Retrovirology en_US
dash.depositing.author Evans, David T.
dc.date.available 2011-09-13T17:46:27Z
dash.affiliation.other HMS^Stipendees - Div of Medical Sciences en_US

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