Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines

Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines

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Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines

dc.contributor.author	Gupta, Sachin
dc.contributor.author	Termini, James M
dc.contributor.author	Niu, Liguo
dc.contributor.author	Kanagavelu, Saravana K
dc.contributor.author	Kornbluth, Richard S
dc.contributor.author	Stone, Geoffrey W
dc.contributor.author	Evans, David T.
dc.contributor.author	Rahmberg, Andrew Richard
dc.date.accessioned	2011-09-13T17:46:27Z
dc.date.issued	2011
dc.identifier.citation	Gupta, Sachin, James M. Termini, Liguo Niu, Saravana K. Kanagavelu, Andrew R. Rahmberg, Richard S. Kornbluth, David T. Evans, and Geoffrey W. Stone. 2011. Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. Retrovirology 8:39.	en_US
dc.identifier.issn	1742-4690	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:5130450
dc.description.abstract	Background Molecular adjuvants are a promising method to enhance virus-specific immune responses and protect against HIV-1 infection. Immune activation by ligands for receptors such as CD40 can induce dendritic cell activation and maturation. Here we explore the incorporation of two CD40 mimics, Epstein Barr Virus gene LMP1 or an LMP1-CD40 chimera, into a strain of SIV that was engineered to be limited to a single cycle of infection. Results Full length LMP1 or the chimeric protein LMP1-CD40 was cloned into the nef-locus of single-cycle SIV. Human and Macaque monocyte derived macrophages and DC were infected with these viruses. Infected cells were analyzed for activation surface markers by flow cytometry. Cells were also analyzed for secretion of pro-inflammatory cytokines IL-1β, IL-6, IL-8, IL-12p70 and TNF by cytometric bead array. Conclusions Overall, single-cycle SIV expressing LMP1 and LMP1-CD40 produced a broad and potent T\(_H\)1-biased immune response in human as well as rhesus macaque macrophages and DC when compared with control virus. Single-cycle SIV-LMP1 also enhanced antigen presentation by lentiviral vector vaccines, suggesting that LMP1-mediated immune activation may enhance lentiviral vector vaccines against HIV-1.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi://10.1186/1742-4690-8-39	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118346/pdf/	en_US
dash.license	LAA
dc.title	Latent Membrane Protein 1 as a Molecular Adjuvant for Single-Cycle Lentiviral Vaccines	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Retrovirology	en_US
dash.depositing.author	Evans, David T.
dc.date.available	2011-09-13T17:46:27Z
dash.affiliation.other	HMS^Stipendees - Div of Medical Sciences	en_US