Fabrication of Myogenic Engineered Tissue Constructs

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Fabrication of Myogenic Engineered Tissue Constructs

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dc.contributor.author Pacak, Christina Ann
dc.contributor.author Cowan, Douglas Burr
dc.date.accessioned 2011-09-21T17:38:19Z
dc.date.issued 2009
dc.identifier.citation Pacak, Christina A., and Douglas B. Cowan. 2009. Fabrication of myogenic engineered tissue constructs. Journal of Visualized Experiments 27. en_US
dc.identifier.issn 1940-087X en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5136968
dc.description.abstract Despite the fact that electronic pacemakers are life-saving medical devices, their long-term performance in pediatric patients can be problematic owing to the restrictions imposed by a child's small size and their inevitable growth. Consequently, there is a genuine need for innovative therapies designed specifically for pediatric patients with cardiac rhythm disorders. We propose that a conductive biological alternative consisting of a collagen-based matrix containing autologously-derived cells could better adapt to growth, reduce the need for recurrent surgeries, and greatly improve the quality of life for these patients. In the present study, we describe a procedure for incorporating primary skeletal myoblast cell cultures within a hydrogel matrix to fashion a surgically-implantable tissue construct that will serve as an electrical conduit between the upper and lower chambers of the heart. Ultimately, we anticipate using this type of engineered tissue to restore atrioventricular electrical conduction in children with complete heart block. In view of that, we isolate myoblasts from the skeletal muscles of neonatal Lewis rats and plate them onto laminin-coated tissue culture dishes using a modified version of established protocols[\(^{2, 3}\)]. After one to two days, cultured cells are collected and mixed with antibiotics, type 1 collagen, Matrigel\(^{TM}\), and NaHCO\(_{3}\). The result is a viscous, uniform solution that can be cast into a mold of nearly any shape and size[\(^{1, 4, 5}\)]. For our tissue constructs, we employ type 1 collagen isolated from fetal lamb skin using standard procedures[\(^{6}\)]. Once the tissue has solidified at 37\(^{o}\)C, culture media is carefully added to the plate until the construct is submerged. The engineered tissue is then allowed to further condense through dehydration for 2 more days, at which point it is ready for \(in\) \(vitro\) assessment or surgical-implantation. en_US
dc.language.iso en_US en_US
dc.publisher Journal of Visualized Experiments en_US
dc.relation.isversionof doi://10.3791/1137 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794293/pdf/ en_US
dash.license LAA
dc.subject cellular biology en_US
dc.subject medicine en_US
dc.subject tissue engineering en_US
dc.subject collagen en_US
dc.subject cellularized matrix en_US
dc.subject electrical conduit en_US
dc.subject hydrogel en_US
dc.subject skeletal myoblasts en_US
dc.subject cardiac en_US
dc.title Fabrication of Myogenic Engineered Tissue Constructs en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Journal of Visualized Experiments : JoVE en_US
dash.depositing.author Cowan, Douglas Burr
dc.date.available 2011-09-21T17:38:19Z
dash.affiliation.other HMS^Anaesthesia-Children's Hospital en_US

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