dc.contributor.author | Broadbent, Kate Mariel | |
dc.contributor.author | Park, Daniel John | |
dc.contributor.author | Wolf, Ashley Robin | |
dc.contributor.author | Van Tyne, Daria Natalie | |
dc.contributor.author | Sims, Jennifer Sung | |
dc.contributor.author | Ribacke, Ulf Erik | |
dc.contributor.author | Cooke, Sarah Volkman | |
dc.contributor.author | Duraisingh, Manoj T. | |
dc.contributor.author | Wirth, Dyann Fergus | |
dc.contributor.author | Sabeti, Pardis Christine | |
dc.contributor.author | Rinn, John L | |
dc.date.accessioned | 2011-11-02T17:45:29Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Broadbent, Kate M., Daniel Park, Ashley R. Wolf, Daria Van Tyne, Jennifer S. Sims, Ulf Ribacke, Sarah Volkman, Manoj Duraisingh, Dyann Wirth, Pardis C. Sabeti and John L. Rinn. 2011. A global transcriptional analysis of Plasmodium falciparum malaria reveals a novel family of telomere-associated lncRNAs. Genome Biology 12(6): R56. | en_US |
dc.identifier.issn | 1465-6906 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:5339087 | |
dc.description.abstract | Background:
Mounting evidence suggests a major role for epigenetic feedback in Plasmodium falciparum transcriptional regulation. Long non-coding RNAs (lncRNAs) have recently emerged as a new paradigm in epigenetic remodeling. We therefore set out to investigate putative roles for lncRNAs in P. falciparum transcriptional regulation.
Results:
We used a high-resolution DNA tiling microarray to survey transcriptional activity across 22.6% of the P. falciparum strain 3D7 genome. We identified 872 protein-coding genes and 60 putative P. falciparum lncRNAs under developmental regulation during the parasite's pathogenic human blood stage. Further characterization of lncRNA candidates led to the discovery of an intriguing family of lncRNA telomere-associated repetitive element transcripts, termed lncRNA-TARE. We have quantified lncRNA-TARE expression at 15 distinct chromosome ends and mapped putative transcriptional start and termination sites of lncRNA-TARE loci. Remarkably, we observed coordinated and stage-specific expression of lncRNA-TARE on all chromosome ends tested, and two dominant transcripts of approximately 1.5 kb and 3.1 kb transcribed towards the telomere.
Conclusions:
We have characterized a family of 22 telomere-associated lncRNAs in P. falciparum. Homologous lncRNA-TARE loci are coordinately expressed after parasite DNA replication, and are poised to play an important role in P. falciparum telomere maintenance, virulence gene regulation, and potentially other processes of parasite chromosome end biology. Further study of lncRNA-TARE and other promising lncRNA candidates may provide mechanistic insight into P. falciparum transcriptional regulation. | en_US |
dc.description.sponsorship | Organismic and Evolutionary Biology | en_US |
dc.description.sponsorship | Stem Cell and Regenerative Biology | en_US |
dc.description.sponsorship | Other Research Unit | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | doi:10.1186/gb-2011-12-6-r56 | en_US |
dash.license | OAP | |
dc.subject | chromosomes | en_US |
dc.subject | DNA | en_US |
dc.subject | epigenetics | en_US |
dc.subject | gene expression | en_US |
dc.subject | gene mapping | en_US |
dc.subject | genes | en_US |
dc.subject | genome analysis | en_US |
dc.subject | genomes | en_US |
dc.subject | human diseases | en_US |
dc.subject | loci | en_US |
dc.subject | malaria | en_US |
dc.subject | mosquito-borne diseases | en_US |
dc.subject | protozoal infections | en_US |
dc.subject | repetitive DNA | en_US |
dc.subject | RNA | en_US |
dc.subject | telomeres | en_US |
dc.subject | transcription | en_US |
dc.subject | virulence | en_US |
dc.title | A Global Transcriptional Analysis of Plasmodium Falciparum Malaria Reveals A Novel Family of Telomere-Associated lncRNAs | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Genome Biology | en_US |
dash.depositing.author | Sabeti, Pardis Christine | |
dc.date.available | 2011-11-02T17:45:29Z | |
dash.affiliation.other | Harvard Medical School, Department of Systems Biology | en_US |
dash.affiliation.other | Broad Institute of MIT and Harvard University | en_US |
dash.affiliation.other | Harvard School of Public Health, Department of Immunology and Infectious Diseases | en_US |
dc.identifier.doi | 10.1186/gb-2011-12-6-r56 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Broadbent, Kate Mariel | |
dash.contributor.affiliated | Sims, Jennifer Sung | |
dash.contributor.affiliated | Wolf, Ashley Robin | |
dash.contributor.affiliated | Van tyne, Daria | |
dash.contributor.affiliated | Volkman, Sarah | |
dash.contributor.affiliated | Duraisingh, Manoj | |
dash.contributor.affiliated | Ribacke, Ulf | |
dash.contributor.affiliated | Wirth, Dyann | |
dash.contributor.affiliated | Rinn, John | |
dash.contributor.affiliated | Park, Daniel John | |
dash.contributor.affiliated | Sabeti, Pardis | |