The Effect of Amnion-derived Cellular Cytokine Solution on the Epithelialization of Partial-thickness Donor Site Wounds in Normal and Streptozotocin-induced Diabetic Swine

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The Effect of Amnion-derived Cellular Cytokine Solution on the Epithelialization of Partial-thickness Donor Site Wounds in Normal and Streptozotocin-induced Diabetic Swine

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dc.contributor.author Bergmann, Juri
dc.contributor.author Koyama, Taro
dc.contributor.author Aflaki, Pejman
dc.contributor.author Robson, Martin C.
dc.contributor.author Hackl, Florian
dc.contributor.author Smith, Charlotte A.
dc.contributor.author Eriksson, Elof
dc.date.accessioned 2011-11-22T15:51:05Z
dc.date.issued 2009
dc.identifier.citation Bergmann, Juri, Florian Hackl, Taro Koyama, Pejman Aflaki, Charlotte A. Smith, Martin C. Robson, and Elof Eriksson. 2009. The effect of amnion-derived cellular cytokine solution on the epithelialization of partial-thickness donor site wounds in normal and streptozotocin-induced diabetic swine. ePlasty 9: 449-459. en_US
dc.identifier.issn 1937-5719 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5350696
dc.description.abstract Objective: The purpose of this study was to determine whether amnion-derived cellular cytokine solution (ACCS) could improve the quality of epithelialization and accelerate closure of dermatome-created partial-thickness wounds in normal and streptozotocin-induced diabetic pigs. Methods: Dermatome-created partial-thickness wounds were sealed with wound chambers in healthy and diabetic pigs and were injected with ACCS. Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12. Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated. Results: The macroscopic appearance of the wounds and speed of healing was similar in all groups at each time point. All wounds were healed by day 6. The epidermis was thicker in the ACCS-treated diabetic wounds than in the controls (140.6 μm vs 82.7 μm on day 12 in diabetic pigs). There were more cell layers (13 vs 7.7) in ACCS-treated diabetic pigs on day 12. The number of rete ridges per 2.5 mm was greater on day 12 in the ACCS-treated diabetic wounds (13 vs 8). There was also a significant increase in the number of rete ridges in ACCS-treated nondiabetic pigs but no difference in epidermal thickness or number of cell layers. Conclusion: In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds. Healthy pigs showed more rete ridges but no difference in thickness of epidermis or number of cell layers on day 12. en_US
dc.language.iso en_US en_US
dc.publisher Open Science Company, LLC en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770806/pdf/ en_US
dash.license LAA
dc.title The Effect of Amnion-derived Cellular Cytokine Solution on the Epithelialization of Partial-thickness Donor Site Wounds in Normal and Streptozotocin-induced Diabetic Swine en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal ePlasty en_US
dash.depositing.author Hackl, Florian
dc.date.available 2011-11-22T15:51:05Z
dash.affiliation.other HMS^Surgery-Brigham and Women's Hospital en_US
dash.affiliation.other HMS^Surgery-Brigham and Women's Hospital en_US

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