ScanImage: Flexible Software for Operating Laser Scanning Microscopes

ScanImage: Flexible Software for Operating Laser Scanning Microscopes

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ScanImage: Flexible Software for Operating Laser Scanning Microscopes

dc.contributor.author	Pologruto, Thomas A
dc.contributor.author	Svoboda, Karel
dc.contributor.author	Sabatini, Bernardo Luis
dc.date.accessioned	2011-11-29T05:03:39Z
dc.date.issued	2003
dc.identifier.citation	Pologruto, Thomas A., Bernardo L. Sabatini, and Karel Svoboda. 2003. ScanImage: Flexible software for operating laser scanning microscopes. BioMedical Engineering OnLine 2: 13.	en_US
dc.identifier.issn	1475-925X	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:5355095
dc.description.abstract	Background Laser scanning microscopy is a powerful tool for analyzing the structure and function of biological specimens. Although numerous commercial laser scanning microscopes exist, some of the more interesting and challenging applications demand custom design. A major impediment to custom design is the difficulty of building custom data acquisition hardware and writing the complex software required to run the laser scanning microscope.Results We describe a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware. Data acquisition and control of laser scanning are achieved through standard data acquisition boards. The entire burden of signal integration and image processing is placed on the CPU of the computer. We quantitate the effectiveness of our data acquisition and signal conditioning algorithm under a variety of conditions. We implement our approach in an open source software package (ScanImage) and describe its functionality.Conclusions We present ScanImage, software to run a flexible laser scanning microscope that allows easy custom design.	en_US
dc.language.iso	en_US	en_US
dc.publisher	BioMed Central	en_US
dc.relation.isversionof	doi: 10.1186/1475-925X-2-13	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161784/pdf/	en_US
dash.license	LAA
dc.title	ScanImage: Flexible Software for Operating Laser Scanning Microscopes	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	BioMedical Engineering OnLine	en_US
dash.depositing.author	Sabatini, Bernardo Luis
dc.date.available	2011-11-29T05:03:39Z
dash.affiliation.other	HMS^Neurobiology	en_US