Ectonucleotidases in Müller Glial Cells of the Rodent Retina: Involvement in Inhibition of Osmotic Cell Swelling

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Ectonucleotidases in Müller Glial Cells of the Rodent Retina: Involvement in Inhibition of Osmotic Cell Swelling

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dc.contributor.author Iandiev, Ianors
dc.contributor.author Wurm, Antje
dc.contributor.author Pannicke, Thomas
dc.contributor.author Wiedemann, Peter
dc.contributor.author Reichenbach, Andreas
dc.contributor.author Zimmermann, Herbert
dc.contributor.author Bringmann, Andreas
dc.contributor.author Robson, Simon Christopher
dc.date.accessioned 2011-11-29T16:10:40Z
dc.date.issued 2007
dc.identifier.citation Iandiev, Ianors, Antje Wurm, Thomas Pannicke, Peter Wiedemann, Andreas Reichenbach, Simon C. Robson, Herbert Zimmermann, and Andreas Bringmann. 2007. Ectonucleotidases in Müller glial cells of the rodent retina: Involvement in inhibition of osmotic cell swelling. Purinergic Signalling 3(4): 423-433. en_US
dc.identifier.issn 1573-9538 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5355303
dc.description.abstract Extracellular nucleotides mediate glia-to-neuron signalling in the retina and are implicated in the volume regulation of retinal glial (Müller) cells under osmotic stress conditions. We investigated the expression and functional role of ectonucleotidases in Müller cells of the rodent retina by cell-swelling experiments, calcium imaging, and immuno- and enzyme histochemistry. The swelling of Müller cells under hypoosmotic stress was inhibited by activation of an autocrine purinergic signalling cascade. This cascade is initiated by exogenous glutamate and involves the consecutive activation of \(P2Y_1\) and adenosine A1 receptors, the action of ectoadenosine 5′-triphosphate (ATP)ases, and a nucleoside-transporter-mediated release of adenosine. Inhibition of ectoapyrases increased the ATP-evoked calcium responses in Müller cell endfeet. Müller cells were immunoreactive for nucleoside triphosphate diphosphohydrolases (NTPDase)2 (but not NTPDase1), ecto-5′-nucleotidase, P2Y1, and A1 receptors. Enzyme histochemistry revealed that ATP but not adenosine 5′-diphosphate (ADP) is extracellularly metabolised in retinal slices of NTPDase1 knockout mice. NTPDase1 activity and protein is restricted to blood vessels, whereas activity of alkaline phosphatase is essentially absent at physiological pH. The data suggest that NTPDase2 is the major ATP-degrading ectonucleotidase of the retinal parenchyma. NTPDase2 expressed by Müller cells can be implicated in the regulation of purinergic calcium responses and cellular volume. en_US
dc.language.iso en_US en_US
dc.publisher Springer Netherlands en_US
dc.relation.isversionof doi:10.1007/s11302-007-9061-3 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2072913/pdf/ en_US
dash.license LAA
dc.subject A1 receptor en_US
dc.subject cellular swelling en_US
dc.subject ectonucleotidase en_US
dc.subject Müller glial cell en_US
dc.subject P2 receptor en_US
dc.subject retina en_US
dc.title Ectonucleotidases in Müller Glial Cells of the Rodent Retina: Involvement in Inhibition of Osmotic Cell Swelling en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Purinergic Signalling en_US
dash.depositing.author Robson, Simon Christopher
dc.date.available 2011-11-29T16:10:40Z
dash.affiliation.other HMS^Medicine- Beth Israel-Deaconess en_US

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