Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation

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Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation

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Title: Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation
Author: Liu, Allen P.; Schmid, Sandra L.; Aguet, Francois; Danuser, Gaudenz

Note: Order does not necessarily reflect citation order of authors.

Citation: Liu, Allen P., Francois Aguet, Gaudenz Danuser, and Sandra L. Schmid. 2010. Local clustering of transferrin receptors promotes clathrin-coated pit initiation. Journal of Cell Biology 191(7): 1381-1393.
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Abstract: Clathrin-mediated endocytosis (CME) is the major pathway for concentrative uptake of receptors and receptor–ligand complexes (cargo). Although constitutively internalized cargos are known to accumulate into maturing clathrin-coated pits (CCPs), whether and how cargo recruitment affects the initiation and maturation of CCPs is not fully understood. Previous studies have addressed these issues by analyzing the global effects of receptor overexpression on CME or CCP dynamics. Here, we exploit a refined approach using expression of a biotinylated transferrin receptor (bTfnR) and controlling its local clustering using mono- or multivalent streptavidin. We show that local clustering of bTfnR increased CCP initiation. By tracking cargo loading in individual CCPs, we found that bTfnR clustering preceded clathrin assembly and confirmed that bTfnR-containing CCPs mature more efficiently than bTfnR-free CCPs. Although neither the clustering nor the related changes in cargo loading altered the rate of CCP maturation, bTfnR-containing CCPs exhibited significantly longer lifetimes than other CCPs within the same cell. Together these results demonstrate that cargo composition is a key source of the differential dynamics of CCPs.
Published Version: doi:10.1083/jcb.201008117
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010081/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:5360612

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