Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation

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Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation

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dc.contributor.author Liu, Allen P.
dc.contributor.author Schmid, Sandra L.
dc.contributor.author Aguet, Francois
dc.contributor.author Danuser, Gaudenz
dc.date.accessioned 2011-12-06T02:12:37Z
dc.date.issued 2010
dc.identifier.citation Liu, Allen P., Francois Aguet, Gaudenz Danuser, and Sandra L. Schmid. 2010. Local clustering of transferrin receptors promotes clathrin-coated pit initiation. Journal of Cell Biology 191(7): 1381-1393. en_US
dc.identifier.issn 0021-9525 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5360612
dc.description.abstract Clathrin-mediated endocytosis (CME) is the major pathway for concentrative uptake of receptors and receptor–ligand complexes (cargo). Although constitutively internalized cargos are known to accumulate into maturing clathrin-coated pits (CCPs), whether and how cargo recruitment affects the initiation and maturation of CCPs is not fully understood. Previous studies have addressed these issues by analyzing the global effects of receptor overexpression on CME or CCP dynamics. Here, we exploit a refined approach using expression of a biotinylated transferrin receptor (bTfnR) and controlling its local clustering using mono- or multivalent streptavidin. We show that local clustering of bTfnR increased CCP initiation. By tracking cargo loading in individual CCPs, we found that bTfnR clustering preceded clathrin assembly and confirmed that bTfnR-containing CCPs mature more efficiently than bTfnR-free CCPs. Although neither the clustering nor the related changes in cargo loading altered the rate of CCP maturation, bTfnR-containing CCPs exhibited significantly longer lifetimes than other CCPs within the same cell. Together these results demonstrate that cargo composition is a key source of the differential dynamics of CCPs. en_US
dc.language.iso en_US en_US
dc.publisher Rockefeller University Press en_US
dc.relation.isversionof doi:10.1083/jcb.201008117 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010081/pdf/ en_US
dash.license LAA
dc.title Local Clustering of Transferrin Receptors Promotes Clathrin-Coated Pit Initiation en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Journal of Cell Biology en_US
dash.depositing.author Danuser, Gaudenz
dc.date.available 2011-12-06T02:12:37Z
dash.affiliation.other 100165 en_US

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