Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors

Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors

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Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors

dc.contributor.author	Spyra, Melanie
dc.contributor.author	Kluwe, Lan
dc.contributor.author	Hagel, Christian
dc.contributor.author	Panse, Jens
dc.contributor.author	Kurtz, Andreas
dc.contributor.author	Mautner, Victor Felix
dc.contributor.author	Demestre, Maria
dc.contributor.author	Nguyen, Rosalyn T.
dc.contributor.author	Rabkin, Samuel David
dc.date.accessioned	2011-12-06T03:19:39Z
dc.date.issued	2011
dc.identifier.citation	Spyra, Melanie, Lan Kluwe, Christian Hagel, Rosa Nguyen, Jens Panse, Andreas Kurtz, Victor Felix Mautner, Samuel David Rabkin, and Maria Demestre. 2011. Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors. PLoS ONE 6(6): e21099.	en_US
dc.identifier.issn	1932-6203	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:5360618
dc.description.abstract	This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free conditions supplemented with epidermal and fibroblast growth factors. From one established human MPNST cell line, S462, cells meeting the criteria for cancer stem cells were isolated. Clonal spheres were obtained, which could be passaged multiple times. Enrichment of stem cell-like cells in these spheres was also supported by increased expression of stem cell markers such as CD133, Oct4, Nestin and NGFR, and decreased expression of mature cell markers such as CD90 and NCAM. Furthermore, cells of these clonal S462 spheres differentiated into Schwann cells, smooth muscle/fibroblast and neurons-like cells under specific differentiation-inducing cultural conditions. Finally, subcutaneous injection of the spheres into immunodeficient nude mice led to tumor formation at a higher rate compared to the parental adherent cells (66% versus 10% at 2.5×10\(^5\)). These results provide evidence for the existence of cancer stem cell-like cells in malignant peripheral nerve sheath tumors.	en_US
dc.language.iso	en_US	en_US
dc.publisher	Public Library of Science	en_US
dc.relation.isversionof	doi:10.1371/journal.pone.0021099	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113907/pdf/	en_US
dash.license	LAA
dc.subject	ensheathing cells	en_US
dc.subject	molecular cell biology	en_US
dc.subject	stem cells	en_US
dc.subject	neurological system	en_US
dc.subject	nerve tissue	en_US
dc.subject	cancers and neoplasms	en_US
dc.subject	neurological tumors	en_US
dc.title	Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	PLoS ONE	en_US
dash.depositing.author	Nguyen, Rosalyn T.
dc.date.available	2011-12-06T03:19:39Z
dash.affiliation.other	100170	en_US