Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors

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Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors

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dc.contributor.author Spyra, Melanie
dc.contributor.author Kluwe, Lan
dc.contributor.author Hagel, Christian
dc.contributor.author Panse, Jens
dc.contributor.author Kurtz, Andreas
dc.contributor.author Mautner, Victor Felix
dc.contributor.author Demestre, Maria
dc.contributor.author Nguyen, Rosalyn T.
dc.contributor.author Rabkin, Samuel David
dc.date.accessioned 2011-12-06T03:19:39Z
dc.date.issued 2011
dc.identifier.citation Spyra, Melanie, Lan Kluwe, Christian Hagel, Rosa Nguyen, Jens Panse, Andreas Kurtz, Victor Felix Mautner, Samuel David Rabkin, and Maria Demestre. 2011. Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors. PLoS ONE 6(6): e21099. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:5360618
dc.description.abstract This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free conditions supplemented with epidermal and fibroblast growth factors. From one established human MPNST cell line, S462, cells meeting the criteria for cancer stem cells were isolated. Clonal spheres were obtained, which could be passaged multiple times. Enrichment of stem cell-like cells in these spheres was also supported by increased expression of stem cell markers such as CD133, Oct4, Nestin and NGFR, and decreased expression of mature cell markers such as CD90 and NCAM. Furthermore, cells of these clonal S462 spheres differentiated into Schwann cells, smooth muscle/fibroblast and neurons-like cells under specific differentiation-inducing cultural conditions. Finally, subcutaneous injection of the spheres into immunodeficient nude mice led to tumor formation at a higher rate compared to the parental adherent cells (66% versus 10% at 2.5×10\(^5\)). These results provide evidence for the existence of cancer stem cell-like cells in malignant peripheral nerve sheath tumors. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0021099 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113907/pdf/ en_US
dash.license LAA
dc.subject ensheathing cells en_US
dc.subject molecular cell biology en_US
dc.subject stem cells en_US
dc.subject neurological system en_US
dc.subject nerve tissue en_US
dc.subject cancers and neoplasms en_US
dc.subject neurological tumors en_US
dc.title Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Nguyen, Rosalyn T.
dc.date.available 2011-12-06T03:19:39Z
dash.affiliation.other 100170 en_US

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