RCAS-RNAi: A Loss-of-Function Method for the Developing Chick Retina

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Title:	RCAS-RNAi: A Loss-of-Function Method for the Developing Chick Retina
Author:	Harpavat, Sanjiv; Cepko, Connie Note: Order does not necessarily reflect citation order of authors.
Citation:	Harpavat, Sanjiv, and Constance L Cepko. 2006. RCAS-RNAi: A loss-of-function method for the developing chick retina. BMC Developmental Biology 6: 2.
Full Text & Related Files:	1402266.pdf (907.7Kb; PDF)
Abstract:	Background: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. Results: We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. Conclusion: This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.
Published Version:	doi://10.1186/1471-213X-6-2
Other Sources:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402266/pdf/
Terms of Use:	This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page:	http://nrs.harvard.edu/urn-3:HUL.InstRepos:5361127